核苷酸结合位点富含亮氨酸重复序列（NLR）基因是植物最大的抗病基因家族。近期研究表明，有的NLR基因可以将病原效应因子攻击的靶蛋白整合到其编码序列中，形成带有整合结构域（ID）的NLR基因——NLR-ID，实现高效的病原识别和抗病。然而迄今为止，这种抗病机制仅在个别NLR基因发现，其在物种间的分布规律和演化模式仍有待探究。本研究拟在前期构建被子植物NLR基因数据库的基础上，通过广泛分析NLR-ID基因在不同物种的存在情况，及ID与病原效应因子的互作潜力，解析融合ID是否是一种普遍存在的NLR抗病机制；通过对NLR-ID基因序列进行演化分析，探究特殊的结构特征和抗病机制对其序列变异模式的影响；通过研究NLR-ID基因在不同生境植物中的分布规律，探索NLR-ID适应不同病原选择压力环境时的演化模式。研究结果有望深化对NLR基因结构变异模式和抗病机制演化的理解，丰富植物抗病基因演化的理论体系。

Nucleotide-binding leucine-rich-repeat (NLR) genes comprise the largest family of plant disease-resistance genes. Recent studies have shown that some NLR genes can integrate the target proteins attacked by pathogenic effectors into their coding sequences to form NLR genes with integration domain (ID), named NLR-ID. The formation of NLR-ID structure enables NLR gene recognize effectors from pathogen more efficient. However, so far, functional NLR genes with NLR-ID structure has only been found in few NLR genes, and its distribution and evolution model among species still need to be explored. Based on the previous construction of angiosperm NLR gene database by our group, this study intends to explore whether fusing ID is a common mechanism for NLR gene function by identification of NLR-ID genes across angiosperm species and examine the potential interaction between IDs and pathogenic effector factors. Then, we will test whether the two functional regions of NLR-ID gene exhibit distinct sequence variation pattern due to their differential role in recognizing effectors. Finally, the distribution of NLR-ID gene in plants in different lifestyles will be analyzed to explore the evolution pattern of NLR-ID genes when adapting to different pathogen selection pressures. The results are expected to deepen the understanding the functional mechanism and evolutionary pattern of the NLR gene family, and provide new insights into the adaptive evolution of plant disease resistance gene.