自体乳糜化脂肪来源间充质干细胞外泌体（ADSCs-Exos）调控皮肤DETC细胞功能促进创面愈合的作用及机制

自体脂肪来源间充质干细胞治疗皮肤损伤创面的机制主要基于脂肪来源干细胞外泌体(ADSCs-Exos)促进血管新生、免疫调节等作用。既往研究发现ADSC可调控T细胞缓解创面炎症反应加快愈合速度，其中分布于表皮组织的树突状表皮T细胞（DETC）在创面愈合中发挥重要作用。ADSCs-Exos可通过激活Wnt /β-catenin 信号传导促进创面愈合，此信号通路可能参与ADSCs-exos调控DETC调节炎症反应促进创面愈合的过程。本项目通过自体乳糜脂肪来源ADSCs-Exos注射皮肤全层创面动物模型创缘，观察注射前后TGF-β、wnt/β-catenin信号通路关键蛋白、DETC创面愈合关键因子的差异性表达，以及抑制wnt3a后,与ADSC-Exos共培养的DETC中几种关键蛋白表达变化，从分子水平明确wnt信号通路在ADSCs-Exos诱导DETC促进创面愈合作用机制，为临床应用奠定理论基础。

The mechanism of autologous adipose-derived mesenchymal stem cells in the treatment of skin wounds is mainly based on the effects of adipose-derived stem cells exosomes (ADSCs-Exos) in promoting angiogenesis and immune regulation.Previous studies have found that ADSCs can regulate T cells to alleviate the inflammatory response of wounds and accelerate the speed of wound healing, among which Dendritic epidermal T cells (DETC) distributed in epidermal tissues play an important role in wound healing.ADSCs-Exos can promote wound healing by activating Wnt /β-catenin signaling, and this signaling pathway may be involved in the process of ADSCs-Exos regulating DETC to reduce inflammation and promote wound healing.This project by autologous chyle fat derived ADSC - Exos full-thickness skin wounds animal models and injection, observed the differences in the expression before and after injection of TGF - beta, WNT/β - catenin signaling pathway key protein, DETC wound healing the key factor, as well as after the inhibition of wnt3a,the changes of several key protein expression.DETC cultured with ADSCs-Exos, definite the mechanism of Wnt signaling pathways in ADSCs -Exos induced DETC promote wound healing from the molecular level, to lay a theoretical foundation for clinical application.