NF-κB信号通路在正畸牙移动过程中对人颌骨骨髓间充质干细胞成骨分化调控机制的研究

错颌畸形是口腔科常见病变之一，不仅影响美观、颜面发育和口颌功能，且易发生龋齿、牙周病和颞下颌关节病，影响心身健康。目前临床针对错颌畸形主要采用正畸矫治，但如何在维持牙周组织健康条件下缩短矫治时间，现在尚无明确的方法。正畸牙在移动过程中伴随着成骨-破骨平衡的重建过程，研究表明炎性微环境对这一过程起重要调控作用。NF-κB信号通路是炎症因子TNF-α下游的关键通路，调控着成骨和破骨分化的进程。我们前期研究发现，调控NF-κB通路的小分子化合物能有效恢复炎症来源牙周膜间充质干细胞的生物学功能，但其对颌骨间充质干细胞的作用并不清楚。本项目拟在前期工作的基础上进一步研究该小分子化合物对颌骨间充质干细胞的作用，同时借助大鼠模型研究正畸过程中，调节NF-κB信号通路改变正畸牙移动速度缩短矫治时间。本研究不仅能揭示NF-κB信号通路在正畸过程中参与骨改建的功能，而且为错颌畸形的临床治疗提供了新的治疗靶点。

Malocclusion is a common oral disease. It affects the appearance, facial growth and stomatognathic functions, in addition, it may lead to caries, periodontal disease and TMD easer. It is a risk factor to people`s physical and mental health. Othodontic treatment is mainly used to treat maiocclusion, but it always needs a long time. How to shorten the treatment time while maintaining the health of periodontal tissue is not clear now. The movement of othodontic tooth is accompanied the process of osteogenesis and osteoclasts balance. Studies have showed that the inflammatory microenvironment is important to this process. NF-κB signaling pathway is a key downstream pathway of inflammatory cytokines TNF-α, and it plays important role in process of bone regeneration. Our previous study found that a small molecule compounds which regulates NF-κB pathway can effectively recover the biological functions of periodontal ligament mesenchymal stem cells from inflammation environment, but the effect to alveolar bone mesenchymal stem cells is not clear. This project intends to do further researches about the NF-κB pathway impact on alveolar bone mesenchymal stem cells on the basis of preliminary studies of the small molecule compounds. Using SD rats animal model to confirm the regulation effects of NF-κB signaling pathway and to the orthodontic tooth movement speed. This study not only reveals that the NF-κB signaling pathways involved in bone remodeling during orthodontic treatment, but also provide new therapeutic targets for the clinical treatment of malocclusion.