最新研究表明，支配胰岛的神经末梢神经递质异常释放启动和参与了一型糖尿病和二型糖尿病的起始。本课题提出工作假说：胰腺内神经系统，作为胰腺分泌的直接调控中心，在一个或多个兴奋性环节发生异常改变，参与糖尿病起始过程。本课题从胰腺内神经系统兴奋性改变入手，将电生理手段引入糖尿病起始研究。研究采用一型糖尿病大鼠模型和二型糖尿病大鼠模型，以胰腺内神经节神经元的兴奋性为主要观察指标，用细胞标记方法，首创的在体胰腺神经元电生理方法，和离体胰腺神经元电生理方法，寻找在糖尿病起始胰腺神经元的兴奋改变方式，特点。通过逐一考察可能造成胰腺神经元兴奋性改变的五个不同环节，确定造成神经元兴奋性改变的具体环节，以及相关的调控机制。提出通过调节胰腺内神经系统兴奋性来阻止糖尿病起始的可行方案。本课题以全新的切入点，研究糖尿病起始过程。本课题的成果，将为糖尿病的预防和治疗，提供新的可能的介入手段，有着重大的理论和现实意义。

Recent studies showed that abnormal neurotransmitter releases from islet-innervating nerve terminals initiated or participated in the onset process of type I and type II diabetes. As such, we developed our project hypothesis: intrapancreatic nervous system, the control center of pancreatic secretion, with abnormal change occurred in one or more segments of the neuronal activity participates in the onset process of diabetes. Focusing on neuronal activity of the intrapancreatic nervous system, we use electrophysiological technique to study the diabetes onset. A type I diabetes rat model and a type II diabetes rat model are used and the activity of pancreatic neurons is monitored with cell labeling method, in vivo intracellular recording method, and in vitro intracellular recording method. Any abnormal activity change will be identified and characterized. Every segment of the activity in pancreatic neurons will be carefully examined to identify any altered segment and to determine the manipulating mechanism. Based on the result of our study, we will outline possible plans to prevent diabetes onset by modulating/manipulating the activity pancreatic neurons.Our project opens an all new entry to the diabetes onset study. The achievement of our study will provide a new possible intervention method to diabetes control and have great theoretical and practical significance.