自噬与帕金森病（PD）密切相关，而平肝益肾法是中医临床治疗PD的基本治法，自噬的“稳态”与中医的“阴阳平衡”存在着相似而微妙的关系。P53蛋白活化AMPK沉默mTOR通路从而促进自噬，保持稳态，抑制神经元细胞凋亡为治疗PD的主要机理；本项目已证明具有调整阴阳平衡的平肝益肾法可通过改善氧化应激反应、神经免疫及细胞凋亡而改善PD。两者是否存在必然联系？因此，本研究将分别以α-synuclein转基因小鼠（体内）和MPP+诱导的SH-SY5Y细胞（体外）为研究对象，采用透射电镜、TUNEL法、细胞转染、实时无标记细胞分析、免疫荧光、免疫印迹法、流式细胞术、RNAi技术等手段，从分子、细胞、组织及动物整体水平多方面探讨P53介导的AMPK/mTOR信号通路在平肝益肾法防治PD中的作用及与自噬和凋亡关系的分子机制，为PD治疗提供新的理论依据。

Autophagy is closely related to Parkinson's disease (PD), and the theory of smoothing the liver and tonifying the kidney is the basic treatment of PD, there is a similar and subtle relationship between autophagy "steady state" and "yin and yang balance". The main mechanism for the treatment of PD is that P53 protein activates AMPK silencing mTOR pathway to promote autophagy, maintain steady state and inhibit neuronal cell apoptosis. This project has proved that smoothing the liver and tonifying the kidney which has the effect of adjusting“yin and yang balance”can treat PD by improving the oxidative stress response, neurological immunity and apoptosis. Are there any links between them? In this study, SH-SY5Y cells induced by MPP + (in vitro) and α-synuclein transgenic mice (in vivo) were used as research targets. Some techniques are used, such as transmission electron microscopy、TUNEL、cell transfection、RTCAxCELLigence、immunofluorescence、western blotting、flow cytometry and RNAi technology. The molecular mechanism of P53-mediated AMPK / mTOR signaling pathway in preventing and treating PD of smoothing the liver and tonifying the kidney and its relationship with autophagy and apoptosis were explored in a wide range of molecular, cellular, tissue and animal levels, which provides a new theoretical basis for PD treatment.