胞外聚合物在嗜热反硝化菌TAD1生物还原去除NO过程中的作用机制研究

生物膜法脱除NOx是近年来备受广泛关注的烟气处理技术。胞外聚合物(EPS)对生物膜的形成，NOx的迁移转化和微生物的活性有重要影响。申请人的研究基础表明，嗜热反硝化微生物（螯台球菌TAD1）的EPS与脱氮效率之间具有相关性。为进一步确定EPS的作用机制，本课题拟采用三维荧光光谱和傅立叶红外光谱技术探索EPS与NO之间的相互作用发生包括络合、配位、吸附和还原等在内的生物、化学和物理的反应，以及由此反应过程所引起的NO的迁移转化、分配、扩散和埋藏等状态的改变；通过微分脉冲伏安法、光谱电化学等技术研究EPS在NO去除过程中氧化还原行为，从分子水平上探讨EPS在电化学还原过程中间体，终产物变化，给出其电子转移机理的直接证据，从而揭示生物法脱除NO的胞外电子传递途径。通过系列研究，深入阐明EPS在嗜热反硝化菌TAD1生物还原去除NO过程中的作用机制，为生物法脱除中温烟气环境中NO提供理论基础。

The biological removal of NO from contaminated gas streams is efficient low-cost NO abatement technologies. Extracellular polymeric substances (EPS) secreted by microorganisms make up an important constituent of labile and semi-labile dissolved organic matter in the environment. Based on our previous research, it was confirmed that there existed a strong correlation between the total EPS content of thermophilic denitrifying bacterium Chelatococcus daeguensis TAD1 and the nitrate removal efficiency. To further confirm the role and function of EPS in the process of bioreduction for NO, three-dimensional fluorescence spectroscopy and infrared spectroscopy will be used to investigate the content of EPS components and their variation as well as the interaction between NO and EPS produced by TAD1, besides, the binging constants were calculated by the modified Stern-Volmer model and the Ryan-Weber model. Meanwhile, differential pulse voltammetry (DPV) and micro-sensor multimeter will be applied to identify the redox properties of the TAD1 EPS and the electrons transfer pathway of NO reduction. Thus, this study can reveal the response mechanism of EPS in the bioreduction of NO and provide theoretical foundation for the removal of NO from the flue gas at thermophilic temperature.