国内外兴奋剂滥用问题日益严重，而现有的兴奋剂检测方法存在预处理复杂、样品处理通量低和抗干扰能力差等缺点，亟需开发快速、高通量的检测方法。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）具有样品制备简单、快速、高耐盐性、定性准确、高通量等优点，尤其适合兴奋剂的高通量初筛。有序介孔材料具有高比表面积、大吸附容量、良好的光电性质、规则可调的孔道结构等特点，因此有望成为一种优异的MALDI基质。本项目拟制备一系列功能化的有序介孔碳材料（如碳、金属、磁性等纳米材料与有序介孔碳的复合材料）作为MALDI基质，提高萃取的效率、选择性和抗基质干扰能力；进一步利用96孔板结合磁分离技术或将功能化有序介孔碳材料直接作为MALDI靶涂层优化样品前处理方法，提高样品处理通量。此项目的成功完成不仅可以满足大型运动会对运动员进行大规模筛查的需要，同时在药物筛选、法医学、临床诊断等领域也具有广泛的应用前景。

The abuse of doping agents is a growing concern worldwide. However, the existing methods for the detection of doping agents suffer significant drawbacks, such as tedious analytical procedure, low sample throughput, and poor tolerance to matrix interferences. Therefore, it is necessary to develop new techniques for high speed and throughput screening of doping agents. In this proposed study, a series of functionalized ordered mesoporous carbon materials (e.g. ordered mesoporous carbon composites with carbon, gold, and magnetic nanomaterials) will be synthesized and tested as new MALDI matrices. The use of functionalized ordered mesoporous carbon materials can be expected to provide better extraction efficiency, higher selectivity and be more tolerance to matrix interference than conventional materials. Additionally, high-throughput in sample preparation will be achieved by a 96-well plate combined with the magnetic separation technique or by using functionalized ordered mesoporous carbon materials as coatings of the MALDI target. This study can potentially satisfy the requirement of large-scale screening of the athletes in large sports games, and it could also be applied in the fields of drug screening, forensic medicine and clinical diagnosis.