无能B细胞（Anergic B cell）是一群能够与自身抗原相结合的自身反应性B 细胞，其免疫的无应答状态是维持免疫自身耐受的重要机制。迄今对无能B细胞在系统性红斑狼疮（SLE）患者中的研究仍知之甚少。我们的前期研究提示SLE中无能B细胞的免疫稳态失衡。为此我们提出假说：SLE中无能B细胞的"免疫无应答"状态被逆转，可能演变成为分泌致病抗体的自身反应性B细胞，作为促进SLE疾病发生发展的危险因素。为验证这一假说，我们拟采用流式细胞术检测无能B细胞在SLE患者中的免疫应答能力，从信号传导通路和细胞间相互作用两个不同角度，探索无能B细胞在SLE中免疫失耐受的机制。试图从分子、细胞及临床患者等多层次阐明无能B细胞免疫耐受失衡在SLE疾病发展中的作用。本研究将为SLE免疫调节紊乱导致无能B 细胞异常活化的机制提供新的思路，为寻求SLE新的治疗靶标作进一步的实践探索和理论铺垫。

Anergic B cells are natural to bind with self-antigen but unable to burst to immune response. The non-response to self-antigen is an important mechanism for the maintenance of immune tolerance. However, the possible role of anergic B cells in Systemic lupus erythematosus(SLE) remain unclear. Our early research found that the anergic state of anery B cells were reversed in patients of SLE. Hence,we hypothesized that the anergic B cells may restore their immune respond ability and then develop to autoreactive B cells in SLE. To verify this hypothesis, we try to assess the anergic state of anergy B cells in patients of SLE to explore the possible mechanisms involved from both intrinsic(cell signal transduction) and extrinsic(cell-cell contact) ways by FACS. In this study, we attempt to demonstrate the possible roles of anergic B cells in the pathogenesis of SLE from moleculars, cells and patients. These results may provide a new mechanism for the abnormal activation of B cells by aberrant immune regulation and envision new possibilities of targeted intervention in the disease pathogenesis.