C18orf1反义长链非编码RNA调控TGFβ/SMAD/JNK信号通路在结直肠癌转移中作用机制研究

长链非编码RNA（lncRNAs）在肿瘤发生发展及转移过程中具有重要作用，反义lncRNA以其特殊结构调控靶基因表达而备受关注。本课题组前期研究提示C18orf1基因的反义lncRNA RP11-701H16.4可能调控C18orf1表达以及TGFβ/SMAD/JNK信号通路，介导上皮间质转化过程（EMT）参与结直肠癌转移，但具体作用机制有待阐明。据此，本课题组拟在前期研究基础上通过体内外实验，重点揭示RP11-701H16.4的生物学功能以及调控靶基因C18orf1的作用机制；阐明RP11-701H16.4调控TGFβ/SMAD和JNK信号通路，介导EMT，参与结直肠癌转移的分子机制。本研究首次阐述了一个新的反义lncRNA- RP11-701H16.4在结直肠癌转移中的分子作用机制，对于筛选可用于靶向治疗和预后评估的分子标志物具有重要的理论意义和潜在的应用价值。

Long non-coding RNA (lncRNA) plays a critical role in the development and progression of malignant tumors. Antisense lncRNA has a special structure and thus has attracted much interest by researchers. Our previous study revealed that antisense lncRNA of C18orf1 gene-RP11-701H16.4 may participate the metastasis of colorectal cancer by regulating C18orf1 expression,TGFβ/SMAD3/JNK signal pathway and epithelial mesenchymal transition (EMT) process,but the detailed mechanism remains unknown.Accordingly, we expect to reveal the biological function of RP11-701H16.4, regulating mechanism of C18orf1 expression, regulating mechanism of TGFβ/SMAD and JNK signal pathway and EMT process. We expect to explore the role of the pathway of RP11-701H16.4- C18orf1-TGFβ/SMAD/JNK-EMT in the metastasis and malignant outcome of colorectal cancer.It is the first time to illustrate the molecular mechanism of a novel antisense lncRNA-RP11-701H16.4 in the metastasis of colorectal cancer, which is valuable in screening new candidate molecular biomarkers for targeted therapy and prognosis prediction.