基于5-HT信号系统探讨电针治疗便秘型肠易激综合征的机制

本项目基于我们长期对肠易激综合征的临床和实验研究，结合目前肠易激综合征发病机制研究进展及5-HT信号系统在其中的重要作用，以5-HT4受体基因敲除杂合子（5-HT+/-, heterozygous, HT）小鼠及子代野生型（widetype, WT）小鼠为研究对象，采用目前国际公认的冰水灌胃法建立IBS-C模型，给予电针治疗，通过免疫组化、高效液相荧光检测法、Western-Blot、RT-PCR、透射电镜等方法观察小鼠结肠黏膜层、黏膜下层神经丛、肌间神经丛、平滑肌组织中的5-HT、5-HIAA、5-HT受体、SERT表达的变化及对结肠转运功能的评价，以期从5-HT信号系统揭示电针治疗IBS-C的作用机制。

The project is based on our long-term clinical and experimental researches about irritable bowel syndrome (IBS), combining the research progress of IBS pathogenesis and the importance role of 5-HT signaling system in it. We choose the 5-HT+/- and widetype mice as objects, modeling IBS-C with the ice pourstomach method which was internationally accepted. After treating mice with electroacupuncture, we observe the expression of 5-HT, 5-HIAA , 5-HT receptors, SERT in mucous layer, submucosa plexus, myenteric plexus and smooth muscle of colon through the method of immunohistochemistry, High-performance liquid fluorescence detection method, Western-Blot, RT-PCR and the transmission electron microscopy. Through the evaluation of expression changes in 5-HT, 5-HIAA , 5-HT receptor, SERT and the transport function of colon, we expect to explore the role of 5-HT signaling system in IBS-C with electroacupuncture.