慢性阻塞性肺病（COPD）膈肌疲劳致死、致残率高，是医疗界广泛关注的热点与难点。COPD膈肌疲劳发病机制尚不清楚，近年研究发现了其可能与泛素蛋白酶体通路（UPP）活性增强相关的线索。我们提出COPD膈肌疲劳的病机是宗气亏虚下陷，前期临床研究表明健脾益肺Ⅱ号能补益宗气，健运脾气，改善COPD膈肌疲劳。本研究拟从目前COPD膈肌疲劳研究的热点"UPP"进行切入，采用RT-PCR、Western Blot等检测方法，围绕炎症失控、氧化失衡、细胞凋亡等多个环节观察对膈肌萎缩信号通路-UPP的影响，阐明健脾益肺Ⅱ号治疗COPD膈肌疲劳的发病机制，对于提高COPD膈肌疲劳治疗的疗效，研发具有膈肌保护作用的中药新药，减轻疾病的经济和社会负担，具有重要意义。

Chronic obstructive pulmonary disease (COPD) diaphragmatic fatigue lethal, high morbidity, hot and difficult is widespread concern in the medical community. COPD diaphragmatic fatigue pathogenesis is still unknown, recent research found may be related to the increased activity of the ubiquitin-proteasome pathway (UPP) related clues. We propose the the diaphragmatic fatigue COPD pathogenesis cases of pectoral qi deficiency subsidence, pre-clinical studies have shown that the Jianpi Yifei II can tonifying pectoral qi and spleen, to improve COPD diaphragmatic fatigue.This study was cut from COPD diaphragmatic fatigue research hot spots "UPP" by RT-PCR,Western Blot detection methods,around the inflammation out of control,the oxidative imbalance apoptosis multiple aspects of observation of diaphragmatic atrophy signal path-UPP,to clarify the molecular mechanism for the treatment of COPD diaphragmatic fatigue Jianpi Yifei II to improve COPD diaphragmatic fatigue treatment efficacy of research and development of traditional Chinese medicine has a protective effect of the diaphragm of new drugs, to reduce the economic and social burden of the disease, is of great significance.