课题组在前期临床实践中证实翘芍方治疗早泄安全有效，发现其能够延长早泄患者的射精潜伏期；最近研究发现，5-羟色胺可能与早泄的发生密切相关，结合最新进展，本研究将进一步探讨翘芍方治疗早泄的可能作用机制。基于连续统一体理论建立早泄大鼠模型，观察大鼠的插入潜伏期、插入次数、射精潜伏期；取大鼠下丘脑，制备下丘脑匀浆，荧光分光光度法检测各标本5-羟色胺的含量；同时观察翘芍方给予早泄患者及健康男性后外周血5-羟色胺的变化，探讨翘芍方改善射精时间治疗早泄的可能作用机制，为临床应用提供新的进一步的理论支持。

It is proved that Qiao-shao Fang was effective and safe in premature ejaculation(PE) treatment in previous clinical practice, and the mixture can extend the ejaculation latency. It was also found in recent study that the 5-HT may have a close relationship with PE, therefore ,based on the ejaculation distribution theory, the rats will be screened and classified into four groups in this study. The intromission and ejaculation latency, intromission frequency of the rats will be observed. The content of 5-HT will be tested in the respective specimens, which were produced by separating from hypothalamus homogenate, will be detected by needle electrode insertion. To find the mechanism of the Qiao-shao Fang in inhibiting the reuptake of 5 - HT and improving the rat ejaculation times, and to provide a new theoretical guidance for clinical treatment.