RNA干扰缺氧诱导因子-1α对肉鸡腹水形成的影响

缺氧诱导因子-1α（HIF-lα)是一种重要的蛋白质调节因子，参与机体缺氧时各种细胞因子表达调控，并在多种疾病中起着关建作用。研究表明：HIF-lα表达与肉鸡 AS 发生密切相关，但关于HIF-lα与肉鸡 AS 冶疗作用机制的研究尚不清楚。因此，本项目以肉鸡为实验动物，利用 RNA 干扰HIF-lα基因进行体内外实验。体外实验是构建慢病毒表达载体—HIF-lα-shRNAi，再转导肉鸡肺动脉内皮细胞（PAEC）中，检测 HIF-1α、VEGF、ET-1表达水平；体内实验是肉鸡体内注射 HIF-lα-shRNAi 重组质粒，以检测血液中HCT、Hb、MDA 等生理生化指标以及组织中 HIF-1α、VEGF、ET-1表达水平。探讨 RNA干扰 HIF-lα在肉鸡 PAEC 中的作用机制，最终阐明 RNA 干扰 HIF-lα表达与肉鸡 AS 发生关系，为实现肉鸡 AS 的治疗提供新的思路。

Hypoxia-inducible factor-la (HIF-lα) is a kind of important proteins regulating factor, involving in regulation expression when the body lack oxygen; and to play a key role in many diseases. Previous studies have shown that HIF-lα expression is closely related to broilers AS, but its research mechanism is not clear that HIF-lα with broilers AS therapy. Therefore, this study takes broilers as experimental animals, and used RNA interference in vivo and in vitro experiments. The vitro experiment is to construct a virus expression vector HIF-lα-shRNAi, and transduction into primitive broilers pulmonary artery endothelial cells (PAEC) under hypoxia, and HIF-1α, VEGF and ET-1 mRNA expression of cell will be detected; The vivo experiment is broilers that HIF-lα-shRNAi plasmids will be taken under the wing intravenously, through detection of biochemical indexes HCT, Hb and MDA etc. in blood, and HIF-1α, VEGF and ET-1 mRNA expression of organization will be detected; explore the mechanism of RNA interference HIF-lα in broilers PAEC, and finally clarify RNA interference HIF-lα expression associated with broilers AS, in order to provide new ideas for the treatment of broilers AS.