盲结构光照明频域交叠成像术研究

结构光照明能够有效增强光学显微术分辨率，它通过融合高、低频谱成分的物体强度信息实现分辨率倍增。但该方法较少用于透明相位物体成像，同时为了获得不同方向频谱信息以及分离多级衍射像，需要精确旋转和移动结构光场。本项目利用随机相位盲结构光照明，拓宽单次照明的频域覆盖方向，利用波长复用、空间复用技术实现不同频域方向、不同衍射级次间频域信息的分离，并采用相位增强方法将相位型物体对光波的相位调制转化为强度调制，最后，利用频域交叠成像迭代算法实现频域信息融合，进而恢复待测物体信息。研究内容含：结构光场构造及复用技术；结构光照明显微术中引入相位增强技术以实现纯相位型物体信息提取的方法；根据盲结构光照明下采集得到的数据，研究求解待测物体信息的方法。该项目将拓展结构光照明显微术应用范围，解决结构光照明在相位型物体测量中的问题；利用盲结构光照明提升采集效率；利用迭代算法简化物体信息求解过程。

Structured illumination is a effective way of resolution enhancement of optical microscopy. However, this technique can only applied to intensity image from amplitude type object rather than phase image from phase type object. And also because of requirement of coverage in frequency domain and separation of different orders, the operation of the technique is complex. So first, phase modulation of the wavefront transmitted from the phase type object is transformed to be amplitude modulation based on phase contrast technique. Then frequency domain coverage direction is expanded base on blind structured illumination of random phase. And wavelength and spatial multiplexing techniques are adopted to realize the information separation of different frequency direction and different diffraction orders. Finally, ptychography iterative engine is used for information fusion. And object transmission function is obtained. The research content includes: formation of structured illumination and multiplexing technique, phase contrast ptychography imaging for phase type object, object information calculation based on blind structure overlap diffraction pattern. The research of the project will enhance the ability of structured illumination microscopy, first solving the problem of phase type object observation with structured illumination, secondly increasing the recording efficiency with blind structure illumination, thirdly simplifying the procedure of frequency domain information fusion with iterative algorithm.