OsWRKY102介导南方水稻黑条矮缩病毒侵染寄主的作用机制研究

南方水稻黑条矮缩病毒（SRBSDV）病是危害我国南方稻区的重要病害，解析其致病机制对水稻抗病育种具有重要意义。前期研究证实SRBSDV的P8蛋白（SP8）能够直接调控并抑制生长素和茉莉酸通路，从而利于病毒自身侵染。然而SP8蛋白是否还通过其他途径介导病毒侵染尚不清楚。我们近期发现SP8与水稻转录因子OsWRKY102转录本2（OsWRKY102.2）特异互作，而OsWRKY102.2又与茉莉酸信号途径OsMYC2转录因子直接互作。本项目拟进一步通过遗传、生化和分子生物学的技术手段，解析SP8与OsWRKY102转录本的差异互作机制，建立SP8-OsWRKY102与茉莉酸信号途径的内在联系，最终阐明OsWRKY102在SRBSDV侵染过程中的作用机制，为全面解析病毒致病机制及寄主抗病途径提供理论依据。

Southern rice black-streaked dwarf virus（SRBSDV）disease is an important rice virus disease in southern China. Investigating the pathogenic mechanism of SRBSDV would provide theoretical supports for rice resistance breeding. Our preliminary studies have revealed that P8 protein encoded by SRBSDV (SP8) can directly manipulate and inhibit the auxin and jasmonic acid pathways, thus facilitating the infection of the virus in rice. However, it is not clear whether SP8 also regulates the other host defense pathways to affect virus infection. Recently, our found SP8 can interact with one transcript of OsWRKY102 (OsWRKY102.2). Further studies showed that OsWRKY102.2 can directly interact with OsMYC2, which is an important transcription factor in the jasmonic acid signaling pathway. Based on these results, this project will focus on the different interaction mechanisms between the SP8 and two OsWRKY102 transcripts through genetic, biochemical and molecular biological technology and methods, elucidate the influence of SP8-OsWRKY102 interaction on the jasmonic acid pathway, and finally clarify the differential role of the two transcripts of OsWRKY102 in response to SRBSDV infection. The study will provide theoretical basis for SRBSDV prevention and control, and contribute to a better understanding of the viral pathogenic mechanism and the defense pathway of rice against virus.