滑膜血管新生是类风湿关节炎（RA）引起骨破坏的重要因素，高迁移率族蛋白1（HMGB1）在诱导血管新生中起关键作用，新近发现沉默信息调节因子1（Sirt1）可作为RA的保护因子负向调节HMGB1诱导的血管新生通路，从而抑制血管新生。已明确补肾解毒通络方临床疗效显著，可抗血管新生，其包含的中药成分对Sitr1有上调作用。鉴此，本课题提出“补肾解毒通络方通过上调Sirt1水平，从而抑制HMGB1诱导的RA滑膜细胞血管新生”假说。拟采用胶原诱导型关节炎大鼠模型以及体外培养人微血管内皮细胞株、RA滑膜成纤维细胞，观察补肾解毒通络方对HMGB1诱导下的血管内皮细胞增殖和血管生成的影响以及该方对Sirt1调控的HMGB1-NF-κB-HIF-1α-VEGF生物信息传递的调节作用。这对于明确中药在RA血管新生中的作用机制，并对指导临床应用和进一步的新药研发具有重要意义。

Angiogenesis of Synovium is an important factor causing bone destruction in rheumatoid arthritis(RA).High mobility group box protein-1(HMGB-1) released plays an key role in the induction of angiogenesis.Recent studies have found that silent information regulator1 (SIRT1) could be negative to the regulation of HMGB1 induced angiogenesis pathway as the protection in RA,thereby inhibiting angiogenesis. BuShenJieDuTongLuo Decoction has been confirmed that it has the clinical curative effect and the inhibiting angiogenesis effect,in which medicine have an up regulation effect on Sitr1.Accordingly, the hypothesis " Inhibition of HMGB1-Induced Angiogenesis of Synovium by the BuShenJieDuTongLuo Decoction via SIRT1 Activation from Rheumatoid Arthritis." is put forward.This study will adopt collagen-induced arthritis rats and human microvascular endothelial cells, RA synovial into fiber cells cultured in vitro, to observe the effect of Bushen Jiedu Tongluo Decoction on vascular endothelial cell proliferation and angiogenesis induced by HMGB1 and the modulatory role on the HMGB1-NF kappaB-HIF-1alpha-VEGF biological informat transmission regulated by SIRT1.It is of great significance to make the mechanism clear of Chinese medicine in RA angiogenesis and to guide the further clinical research and development of new drugs.