马麝是我国特有的麝类动物资源，是麝香的主要生产动物之一。近几年来，甘肃马兴隆山马麝养殖场的马麝种群中流行一种由病毒引起的以多组织和脏器广泛出血坏死为特征的急性、高度致死性传染病，经济损失惨重。但该病的致病病毒至今尚未确定，为此，本研究拟选择不同动物源性的原代和传代细胞及鸡胚等病毒培养材料，对该病毒进行分离，进而系统鉴定该病毒的形态结构特点、理化学特征及培养特性，并对其核酸序列和基因组结构进行分析研究。同时针对病毒的致病性及免疫原性开展相应的基础研究工作和流行病学方面的调查工作，以期为该病的致病和免疫机理及预防和控制措施的研究奠定基础。

The alpine musk deer only live in the highlands in the western region of China. is the largest cultivation base in China. In recent years, a kind of acute infectous disease is spreading amongst alpine musk deer in Xinglongshan National Nature Reserve. The characteristics of the infectious disease are extensive hemorrhage and necrosis in trachea, heart, lung, liver, spleen and other visceral organs. Our preliminary invastigations show that the disease is a highly deadly infectious disease caused by a RNA virus with its etiology characteristics and pathogen need to be further elucidated. So, this study will choose different animal cells and chick-embryo to isolate and cultivate the virus. Then its morphological characteristics, physiochemical characteristics and cultural traits will be identified. Based on these, the nucleic acids sequences of virus will be amplified by PCR and the analysis on structure and function of genome will be annotated. Meanwhile, the studis on viral pathogenicity, immunogenicity and epidemiological investigations of Viral Hemorrhagic Disease on Alpine Musk Deer will be implemented. This study will provide a theoretical basis to prevent and control this disease and lay the foundation for exploring the pathogenic and immune mechanism of the disease.