糖尿病并发抑郁症（DD）继发于糖尿病，发病率约占糖尿病患者的30%，其发病机制至今未明。近年研究表明，N-甲基-D-天冬氨酸受体（NR）是一种谷氨酸敏感性离子通道受体，其相关通路可调控海马神经元调亡、突触重塑及5-羟色胺系统，与HPA轴亢进致海马损伤密切相关。本项目根据课题有关"DD大鼠HPA轴亢进，皮质醇（COR）、促肾上腺皮质激素释放激素（CRH）水平升高，海马神经元结构可塑性降低"的研究结果，结合中医病机及现代医学对DD发病机理的认识，拟采用膜片钳、流式细胞、WB及RT-PCR等技术，从整体-细胞-分子三水平、形态-功能双角度，探明"COR、CRH水平升高，激活NR相关信号通路，介导海马损伤，导致DD发生"的分子机制，揭示DD海马损伤的特点与规律，以及"虚、瘀、郁"病机与其发生机制的相关性，并采用滋阴益气、化瘀解郁治则的中药复方干预反证，为DD发病机制的研究及防治提供新的思路与靶点。

Diabetes with depression (DD) secondary to diabetes, its incidence accounts for approximately 30% suffering from diabetes, the pathogenesis is still unknown.In recent years, studies have shown that N-methyl-D-aspartate receptor (NR) is a glutamate-sensitive ion channels receptor, the pathways can regulate the apoptosis of hippocampal neurons, synaptic remodeling and 5 - serotonin system, closely related to the HPA axis hyperactivity induced hippocampal damage.This project based on the topics related to "The DD rats with HPA axis hyperactivity, cortisol (COR)and corticotropin-releasing hormone (CRH) elevated levels of hippocampal neurons structural plasticity reduce", intending to combine the understanding about DD pathogenesis of TCM Pathogenesis and modern medicine, using patch clamp, flow cytometry, WB and RT-PCR technology, to prove the molecular mechanism about"COR, CRH level rise, through the NR related signaling pathways, mediated hippocampal damage, leading to the DD occurs", to reveal the characteristics of the DD hippocampal damage morphology - function, pathogenesis and correlation between "asthenia", "stasis" and "Yu" from the whole - cell - molecular level, dual-angle from shape - function.To provide new ideas and targets for studies of pathogenesis and prevention about DD, using intervention of nourishing yin,benefiting vital energy, circulation and Jieyu.