海洋肠杆菌科细菌检出率较高，该菌及抗性基因传播对公众有很大健康威胁。其中"超级耐药基因"NDM-1能随人群迁移而快速传播。本研究对3个海水浴场采样，筛选肠杆菌并检测环境因子。通过细菌培养、药敏实验、RealtimePCR、基因及酶检测，建立基于种群、基因、mRNA及酶水平的多层级方法,考察耐药菌及抗性基因的时空变化规律及环境影响机制。明确优势耐药菌、ESBLs菌、耐碳青霉烯肠杆菌及抗性基因等流行状况；探讨环境对抗性基因转移的影响。对人群粪便细菌进行转化及接合实验、ERIC-PCR方法分析环境及人群肠杆菌的同源性及抗性基因的传播。培养多重耐药菌，评估其环境风险。该研究前期建立了成熟的环境检测及抗性基因研究方法。从紫外线、富营养因子等角度探讨耐药影响机制；进行blaNDM及人群研究；具有较好的创新性。该研究可为规避浴场耐药菌的人群风险提供理论及实际依据,为抗性基因的环境及人群研究提供基础资料。

The detection rate of antibiotic resistance Enterobacteriaceae is high in marine environment. It would be a great threat for the public with the spread of bacteria and resistant genes. In particularly, there are trends of the spread in floating population for "Super resistance gene" NDM-1 of carbapenemase resistant Enterobacteriaceae.Three bathing beaches were selected for study on the resistant characteristic from the perspectives of time and space; The drug sensitivity test,Realtime-PCR, plasmid transformation and bacterial conjugation experiments will be used to establish the multi-level approach for the study on influencing mechanism ,which will detect it from bacterial communities , antibiotic resistance genes , mRNA and expression of the enzyme.We attempt to clarify the prevalence of ESBLs,CRE,and dominant bacterium on beaches and the ability of transfer of ARGS in different environment.The feces will be collected from the high risk population and the control group to investigate the possible spread of ARGS between the beaches and populations, and the homologous analysis of bacterium will be analyzed by ERIC-PCR. The human sourced Enterobacteriaceae which have high homology with environment,the Enterobacteriaceae with dangerous ARGS will be cultured and the environmental risk will be predicted.It is innovative that the mechanism of resistance were explored from the UV rays, from organics and other environmental factors, blaNDM genes and bacteria of the populations which were investigated.The study can provide theoretical and practical basis for avoiding the risk of population transmission of antibiotic-resistant bacteria and ARGS on bathing beaches, and provide basic data for the research of antibiotic resistance Enterobacteriaceae and populations on the bathing beaches.