水稻花粉萌发孔是花粉外壁中的一种特化结构，其正常发育对维持外壁完整性、花粉完全成熟和花粉萌发来说非常重要，然而水稻萌发孔发育的分子机制尚不清楚。此外，GDSL脂肪酶基因在水稻花粉发育中的作用很不清楚。我们发现一个新颖的水稻雄性不育突变体，除带有一些常见的不育特征外，其特别之处在于：花粉萌发孔孔盖丢失。图位克隆表明这个萌发孔孔盖丢失基因OsAOM为一个GDSL脂肪酶基因，基因突变（T→C）使得相应氨基酸由Cys变为Arg。本项目打算用光镜、电镜等对突变体的表型进行详细观察；用遗传互补验证该基因的功能；用原位杂交、Q-PCR、亚细胞定位等手段，研究该基因的表达模式；分析OsAOM蛋白结构和进化；检测该基因野生型蛋白产物和突变蛋白的脂肪酶活性。最终阐明OsAOM基因在花粉萌发孔发育中的作用机制。本研究将有利于认识水稻花粉萌发孔发育的分子机制，有利于认识GDSL脂肪酶基因在花粉发育中的作用。

Rice pollen aperture is a specialized structure in exine and is very important for exine keeping integrity, pollen maturing completely and pollen tube germinating. However, the little is known about the molecular mechanism underlying the aperture development in rice. In addition, the role of GDSL-lipase gene in pollen development is extremely unclear. We found a novel rice male sterility mutant displaying an unique phenotype: pollen aperture operculum missing (AOM), also presenting some familiar sterile character such as: white anther,small and abnormal pollen, and no starch accumulation. By mapbased cloning, we have isolated a GDSL-lipase gene as the candidate gene of OsAOM(aperture operculum missing),and found a T→C mutation resulting a amino acid substitution of Cys→Arg in the mutant of osaom. We plan to characterize the mutant detail phenotype by using light and electron microscope; validate the function of OsAOM gene by genetic complement experiment; investigate the expression pattern of OsAOM gene by RNA in situ hybridization, Q-PCR and subcellular localization;analyze the protein structure of this GDSL-lipase and perform the phylogenetic analysis; and testify the lipase activity of OsAOM and osaom protein. At last, we expect to clarify the function of OsAOM gene in rice aperture development. After this research, we may get the proof of OsAOM gene controlling the aperture development, approach to the molecular mechanism of aperture development and learn more about the GDSL-lipase role in pollen maturation.