本项目在完成广东省社会发展攻关基金重点引导项目的基础上，应用含有缓释神经营养素-3 (NT-3) 的多通道圆柱体明胶/丝素蛋白导管支架，吸附维甲酸(RA)预诱导的神经干细胞(NSCs)，在体外构建具有功能的人工神经网络样导管，并研究三维空间条件下NT-3通过哪种细胞信号通路介导RA预诱导的NSCs更多地分化为具有形成突触潜能的神经元。再将此神经网络导管移植到动物脊髓损伤处，观察其能否修复受损伤的神经网络，促进受损伤的脊髓功能和结构的修复，研究其保护受损伤神经元，促进再生神经形成髓鞘及恢复瘫痪肢体自主运动的作用。最终通过这种能够缓释NT-3、在结构上高度仿生的并且能安全应用于体内脊髓损伤修复的导管支架，为进一步临床前研究和应用提供一种新型功能化组织修复材料和新的治疗策略

Upon completion of the project of Guangdong Natural science foundation,this project based on the application containing the release neurotrophin -3 (NT-3) multichannel cylinder gelatin / silk fibroin stent adsorption retinoic acid (RA) pre-induced neural stem cells (NSCs), in vitro functional artificial neural network-like tube, and to study the three-dimensional space under NT-3 signaling pathway by which cell-mediated RA-induced NSCs more pre-to form synapses with the potential to differentiate into neurons. This neural network-like tube and then implanted into animal spinal cord injury at the focus animals were observed after transplantation repair injured spinal cord neural network structure and function, protect damaged neurons and promote regeneration of nerve myelin formation and the restoration of the autonomy of paralyzed limbs movement and other indicators. Eventually developed a way to release NT-3, the structure of highly biomimetic for spinal cord injury repair and can be safely used in the body for further pre-clinical studies and applications of a new functional tissue repair materials and new treatment strategy.