呼吸道合胞病毒（RSV）是导致下呼吸道感染的常见病原体，前期十一五课题研究证实扶正解毒化瘀方可减轻RSV感染老年肺炎的症状，控制疾病发展，但机理尚不明确。在RSV感染早期，PI3K/Akt通路在RSV激活的多条信号通路中发挥中心作用，可通过抑制细胞凋亡，促进融合性病变形成，有利于病毒复制。中药复方发挥药效时间恰是感染早期，中医认为RSV感染早期属于风温肺热病的正虚热毒瘀肺证，由此提出“风温肺热病正虚热毒瘀肺的时期正是RSV融合病变形成的复制期”假说。本课题以药效作用为基础、有效部位群为整体、体内外成分为引导，运用化学计量学、药效学和谱学相结合的方法，建立扶正解毒化瘀方总方、各有效部位、有效部位配伍组方体内外指纹图谱，同时观察其通过PI3K/Akt通路对细胞凋亡和炎性反应的影响，阐明扶正解毒化瘀方抑制RSV融合性病变形成的物质基础和作用机制，为中医药防治RSV带来更广阔的应用前景。

Respiratory syncytial virus (RSV) is a common pathogen that leads to lower respiratory tract infection. Preliminary study supported by National TCM Project Application in the 11th Five-Year Period confirms that Fuzheng Jiedu Huayu decoction can relieve the symptoms and control the progression of RSV infection in aged pneumonia patients. But the mechanism is not clear. In the early stage of RSV infection, the PI3K/Akt pathway plays a central role in signaling pathways activated by RSV. It causes the virus replication by inhibiting cell apoptosis and promoting the integration of lesion formation.The action time of Chinese herbal compound is at the early stage of virus infection. It is believed by traditional Chinese medicine that the early stage of RSV infection belongs to the deficiency of heat toxin stasis lung syndrome of wind-warm disease with lung heat, which puts forward the hypothesis “the phase of deficiency of heat toxin stasis lung syndrome in wind-warm disease with lung heat is the replicative phase of RSV fusion lesions”. This study intends to establish the compound prescription of Jiedu Huayu and the fingerprint of effective fractions of the overall composition in vitro and in vivo on the basis of pharmacodynamics, effective fractions and ingredients. At the same time , material basis and mechanism of action of Fuzheng Jiedu Huayu Decoction of anti RSV are illustrated through observing PI3K/Aktpathway on cell apoptosis and inflammatory reaction. A wider application prospects will be brought to the prevention and treatment of traditional Chinese Medicine on RSV.