基于“肠道菌群-肠上皮细胞”途径研究艾灸重建克罗恩病肠道免疫稳态的作用机制

克罗恩病(Crohn’s disease，CD) 是具有易感基因的人群对不良的环境因素产生过激的免疫应答，从而导致累及消化道为主的慢性炎症性损伤。肠道菌群已被证实对肠道免疫系统的发育和激活具有重要作用, 肠道菌群变化可能诱发或加重CD，而“肠道菌群-肠上皮细胞” 是重建CD肠道免疫稳态的重要途径。目前，调节肠道菌群结构，抑制和缓解肠道炎症，重建肠道免疫稳态成为治疗CD的一种新方式。研究表明，艾灸能够修复细胞间紧密连接，改善CD结肠上皮屏障结构与功能。本研究以肠道菌群与肠上皮细胞和肠道免疫系统的相互作用为切入点，紧扣CD发生发展过程中的两个关键环节，即“肠道菌群失调”与“Th17/Treg细胞失衡”，运用16S rDNA基因片段PCR、RFLP分析技术、1H-NMR代谢组学技术等多种生物技术手段，基于“肠道菌群-肠上皮细胞”途径进一步揭示艾灸重建克罗恩病肠道免疫稳态的作用机制。

Crohn's disease (CD) is a kind of chronic inflammatory injury of digestive tract which caused by ultra-immune response of ill-natured environmental on people has susceptibility genes. Intestinal flora has been confirmed plays an important role in the development and activation, the changes of intestinal flora may induce or aggravate CD. "Intestinal flora-intestinal epithelial cells" is an important way of immunity homeostasis reconstruction. Currently, adjust the structure of intestinal flora, restrain and reduce the intestinal inflammation, reconstruction of the intestinal immune homeostasis become a new way for the treatment of CD. Research shows that moxibustion can repair the connection between cells, improve the structure and function of epithelial barrier on CD. our study focusing on intestinal flora and intestinal epithelial cells and the interaction of intestinal immune system, stick to two key links of "intestinal flora imbalance" and "imbalances of Th17 / Treg cells " in the process and development on CD, using 16s rDNA gene segment, PCR, RFLP analysis technics, 1H-NMR metabolomics to reveal the regulation mechanism of reconstruction immunity homeostasis of moxibustion on Crohn's disease based on the pathway of "Intestinal flora-intestinal epithelial cells".