肝再生是机体正反两方面因素调控下的复杂过程。目前，对肝再生研究主要集中在正调控机制上，而对肝再生负调控机制的研究相对较少，肝再生终止以及再生肝量精细调节等问题至今仍不清楚。我们在进行大鼠肝再生研究时发现，PLCG2与肝再生关系密切；根据以往研究并结合我们的生物信息学分析，认为PLCG2在肝再生中可能依赖PKCD激活p38和/或JNK途径促进细胞凋亡。本项目以此科学假设为主线，以腺病毒感染体外培养的大鼠再生肝肝细胞为切入点，通过基因添加和干涉等方法研究肝细胞中PLCG2的表达，用Hoechst/PI双染、流式细胞术等方法检测PLCG2对细胞凋亡和增殖的影响、用信号通路抑制剂、qRT-PCR、Western Blot 等方法检测上述材料中PLCG2及下游信号通路相关基因和蛋白的变化，以及信号通路改变与细胞凋亡之间的关系，从而解析其作用及作用途径，以期为后续体内研究和临床应用积累资料。

Liver is one of the few organs which have the capacity to regenerate rapidly after injury. Around one week after 70% partial hepatectomy, the liver recovers to its original mass and volume. Liver regeneration (LR) is a complex process under precise regulation from numerous proliferative and inhibitory factors. At present, many studies mainly focus on the triggering and proliferative mechanism involved in LR. And many factors (hormones, growth factors, cytokines) playing important roles in initiation and progression of LR have been extensively studied. Inhibitory regulation mechanism, such as LR termination, apoptosis of excessive hepatocytes and maintenance of normal liver mass, remain to be studied. In our previous work on proteomics of rat liver regeneration, we found that phospholipase C-γ2 (PLCG2) has a key protein for liver regeneration; bioinformatics analysis showed that PLCG2 possibly induces cell apoptosis during liver regeneration through PKCD-dependent p38 and JNK cascades activation. Taking this scientific hypothesis as the main line, we firstly control the expression change of PLCG2 in the isolated primary hepatocytes from rat regenerating liver through gene addition and gene interference, and then detect the influence of PLCG2 on cell apoptosis and proliferation using various approaches including Hoechst/PI double-staining, flow cytometry and so on. Meanwhile, the expression changes of PLCG2 and PKCD-activating signaling-related components will be detected to analyze its possible its possible pathway by qRT-PCR, Western Blot, administration of signal pathway inhibitors and so on. This study will be helpful in unraveling the role and action mechanism of PLCG2 in liver regeneration, which prepares good conditions for the continued in vivo study and its clinical application.