饲料安全影响瘤胃菌群的稳定，从而影响反刍动物的健康。本项目是从我们前期工作获得，并证明具有显著改善苜蓿青贮品质的微生物基础上，进行的动物性实验，通过采用免培养的16SrDNA序列分析、DGGE技术和real-time PCR技术，了解不同季节舍饲条件下多浪羊瘤胃细菌多样性变化规律；再通过饲喂添加来自骆驼刺青贮中的微生物及其微生物组合的饲料，分析多浪羊瘤胃细菌的结构和主要纤维降解菌数量的变化，推断骆驼刺青贮中优势乳酸菌、胶红酵母及其相互作用对多浪羊瘤胃微生物的影响，明确瘤胃主要微生物对骆驼刺青贮中优势乳酸菌和胶红酵母的反应，确定瘤胃微生物对饲料微生物是否具有适应性，达到判断饲料微生物的安全性目的，为开发利用微生物饲料资源提供依据。

The objective of the study is to judge the feed microbial safety and provide the basis for the development and utilization of microbial feed resource.Feed safety impact Stability of bacterial communities in rumen,and the latter affect ruminant health. These lactic acid bacteria and Rhodotorula mucilaginosa being used for animal experiments in this project can significantly improve the quality of alfalfa silage.And the results have been confirmed during our preliminary work. The Project will be done by adding lactic acid bacteria or R. mucilaginosa or their mixed microorganisms in feed to feed duolang sheep,then gets the rumen fluid of these sheep to Analysis the change of 16SrDNA sequences of the rumen bacterial with unculture bacteria ,DGGE and real-time PCR technology.Find out the difference of rumen bacterial diversity & number of main fiber-degrading bacteria with seasonal and feeds variation.Then,infer the effect of mainly lactic acid bacteria,or R. mucilaginosa or their mixed microorganisms on rumen bacteria.Judging the rumen microorganisms whether adapt to feed microbial and the latter security.