回肠膀胱（Bricker）术需截取患者自身健康回肠用作尿液流出通道，破坏肠道导致并发症发生率极高。申请者构建组织工程管替代术中所需回肠，对该术式进行改良，已通过动物实验证实可行。但组织工程管植入后存在继发感染、难存活等问题，限制了进一步临床应用。有研究发现天然免疫识别分子SP-D的基因多态性与尿路感染存在着密切联系，SP-D作为抗感染的天然免疫屏障发挥着重要作用。本研究拟通过将转染SP-D基因的脂肪干细胞（ADSCs）与ePTFE支架材料构建组织工程复合物，代替Bricker术中需要的回肠；采用PCR、Western-Blot等方法检测SP-D基因和产物的表达情况，通过细菌凝集试验、体外抑菌圈试验等检测基因增强型ADSCs的抗感染能力，不同时间节点取材行HE、IHC等检测复合物植入后上皮细胞生长情况，探讨基因增强型组织工程管改良Bricker术的效果，为进一步临床应用奠定基础。

Bricker’s surgery use the patient's own healthy ileum as urine outflow channel, leading to the high complication rate related to intestinal injury. We tried a tissue-engineered conduit for the replacement of ileum during Bricker’s surgery to limit the complications, which has been confirmed by animal experiments. However, this new technique could not applied on patients, the infection after implantation limiting the clinical application. Some studies suggested that gene polymorphisms of innate immune recognition molecules SP-D involved in urinary tract infection, and as an innate immune barrier, SP-D plays an important role during pathogen infection. In this study, we attempt to construct a tissue-engineered conduit using ADSCs with SP-D gene and ePTFE scaffold, used as urine outflow channel in Bricker’s surgery, to avoid the intestinal complications, using PCR, Western-Blot to detect the SP-D gene and protein expression, test the anti-infection function of the gene enhanced ADSCs and conduit by bacterial agglutination tests and inhibition zone tests, test the epithelial cell proliferation ability by HE and IHC tests, to explore effect of gene-enhanced tissue-engineered conduit in Bricker’s surgery and may form the basis for further clinical application.