清热中药消除禽源大肠杆菌耐药性的分子机制

当前兽医临床细菌耐药现象非常严重，使细菌性疾病防治难度不断加大，耐药现象已成为全球医学亟待解决的问题。本试验选取禽源性大肠杆菌作为研究对象，检测其耐药性，探讨黄连、白头翁、金银花、牡丹皮等清热类中药对大肠杆菌耐药性的消除（逆转）作用。通过对主要耐药基因、主动外排系统外输泵基因、调控基因的扩增测序和mRNA表达量检测，R质粒消除，耐药功能蛋白，生物膜检测等方面进行研究，并观察消除后细菌耐药性的转归规律，系统地揭示中药消除大肠杆菌耐药性的分子生物学机制，筛选中药耐药性消除剂，为解决细菌耐药问题提供理论基础。本研究立足于我国对中药研究的传统优势及强大的市场需求，满足于公共卫生及食品安全的迫切要求，对治疗临床上由耐药菌导致的疾病和阻断细菌耐药性的传播具有十分重要的意义，同时对丰富中兽医理论具有积极的作用。

The current tough situation of bacterial drug resistance makes the controlling work on bacterial diseases more difficult and becomes one of the global issues of common concern. In this proposal, the drug resistance of Escherichia coli strains from avian origin will be monitored to study the drug resistance eliminating effects of Pulsatilla Chinensis and other Chinese herbals, such as Coptis Chinensis and Scutellaria Baicalensis. The resistance gene, active efflux system gene and regulatory gene will be amplified and sequenced, mRNA expression, R plasmid elimination and resistance-related protein will be detected before and after the treatment with Chinese herbals, so as to explore the molecular mechanism of Chinese herbs on the elimination of drug resistance. The present study integrate the advantage of traditional Chinese medicine with the urgent market demand, searching for the green natural ways for public health and food safety. It has very important significance to control the drug resistant bacteria diseases and has a positive role on enriching the theory of traditional Chinese veterinary medicine.