肿瘤转移是胆囊癌患者的主要死亡原因，外泌体在肿瘤转移中扮演重要角色，而外泌体在胆囊癌转移中的作用机制尚不清楚。我们前期研究发现LncRNA与胆囊癌转移密切相关，并从高低转移潜能胆囊癌细胞外泌体中筛选出差异表达的LncRNA-LINC00239，进一步证实富含LINC00239的外泌体显著促进胆囊癌细胞侵袭转移，生物信息学预测LINC00239与miRNA-200a结合影响侵袭转移相关基因。据此我们提出假说：高转移潜能胆囊癌细胞通过外泌体将LINC00239传递给低转移潜能胆囊癌细胞，从而增强后者的转移能力。为此本项目拟进行：（1）明确外泌体中LINC00239对胆囊癌细胞侵袭转移的作用；（2）揭示LINC00239调节侵袭转移的分子机制；（3）探讨外泌体中LINC00239作为胆囊癌病人复发转移标志物及治疗靶点的可行性，为胆囊癌转移的早期诊断和靶向治疗提供新思路。

Tumor metastasis is the main cause of death in patients with gallbladder cancer. Exosomes play an important role in tumor metastasis. However, the role of exosomes in the metastasis of gallbladder carcinomas remains unclear. Our previous study found that LncRNA was closely related to the metastasis of gallbladder carcinoma and screened out differentially expressed LncRNA-LINC00239 from the exosome from highly and lowly metastatic potential gallbladder carcinoma cells. It was further confirmed that exosomes rich in LINC00239 significantly promoted the invasion and metastasis of gallbladder carcinoma cells. Bioinformatics prediction: LINC00239 combined with miRNA-200a and affects metastasis related gene. Based on this, we hypothesize that high metastatic potential gallbladder carcinoma cells deliver LINC00239 to low metastatic potential gallbladder carcinoma cells via exosomes, thereby enhancing the latter's metastatic potential. To this end, the project is planned to: (1) clarify the role of LINC00239 in exosomes in invasion and metastasis of gallbladder carcinoma cells; (2) reveal the molecular mechanism of LINC00239 in regulating invasion and metastasis; (3) to investigate the feasibility of LINC00239 in exosomes as a marker or therapeutic target of recurrence and metastasis in gallbladder carcinoma. These provide new ideas for early diagnosis and targeted therapy of gallbladder carcinoma metastasis.