食源性诺如病毒（NV）和轮状病毒（RV）对人类健康产生的危害已经引起了全球的广泛关注。然而，现有检测方法在灵敏度和现场多重可视化方面仍面临巨大挑战。本课题拟首先利用包含适配体序列的核酸探针代替传统抗体，全面特异地识别病毒分子，并通过Nicking酶恒温扩增反应（NEAR）对识别信号迅速放大，解决灵敏度低的问题；其次，提出利用发射光信号叠加同时分析多目标的可视化检测策略，结合金-量子点荧光能量共振转移（FRET）体系，设计可由信号扩增所得核酸分子特异性开启的荧光探针，达到One-pot无需开盖多重可视化分析的目的。该方法具有灵敏、多重、可视化的特点，为食源性病毒检测提供了新思路；且可被拓展应用于食品安全领域多类目标分子的现场快速分析，为食品污染物监控、食源性疾病预防控制提供了新技术。

Food-borne Norovirus (NV) and/or Rotavirus (RV) virus infection has been a serious problem threatening human health and thus drawn global concern. The existing methods related have been challenged by the increasing needs in high sensitivity and multiplex in-field visual detection. To address these two problems, we first employ aptamer contained molecular probe instead of conventional antibody to discriminate interested virus particles, and amplify the signals produced promptly via nicking enzyme amplification reaction (NEAR). Next, we proposed a novel strategy to detect multiple targets simultaneously and visually by analyzing emission lights blending signals. In this design, gold-quantum dots complex is employed and functionalized into fluorescent probe targeting virus-specific reporters obtained from NEAR amplification. Once specific reporter is present, the fluorescent signal will be “turn-on”. And if there are multiple targets, a blending of emission lights will be observed. With this, visual multiplex analysis can be achieved in one-pot without uncapping operation. To sum up, this technique is of high sensitivity and multiplex visual analysis capability, providing attractive new ideas for food-borne virus detection. It can also be expanded to target many other food safety related biomolecules to fulfill the needs for food contaminations monitoring and food-borne disease prevention and control.