从LncRNA对转录因子EGR2的调控对RA寒证机制的进一步研究

寒热辨证在RA中医诊治中发挥重要作用,RA寒证有其特征性生物机制。课题组前期工作显示，RA寒证的影像学及以转录因子Egr2介导的炎性信号通路有其特征。长链非编码RNA（LncRNA）作为转录调控重要角色参与RA炎性过程。基于此本研究从LncRNA与Egr2的调控进一步对类风湿关节炎寒证生物学机制进行研究；方法：（1）临床研究：纳入RA寒证、热证患者，测定临床资料及免疫学指标，并采集外周血及滑膜，测定LncRNA HOTAIR，分析与临床表征的相关性；（2）细胞学水平研究：制备外周血CD4+T细胞及滑膜成纤维细胞，利用RNAi方法，研究LncRNA HOTAIR及Egr2为核心的炎性信号通路的相互调控作用；（3）动物实验：基于前期课题复制CIA寒证疾病证候复合模型，测定LncRNA HOTAIR及Egr2为核心的炎性信号通路的相互调控作用；并通过温经通络法干预后变化进行验证。

The syndrome of cold and heat plays an important role in the diagnosis and treatment of RA. The cold syndrome has its characteristic biological mechanism. The preliminary study shows that there are characteristics of the imaging of RA cold syndrome and the inflammatory signal pathway mediated by transcription factor Egr2. Long-chain non-coding RNA (LncRNA) is involved in the inflammatory process of RA as an important role in transcriptional regulation. Based on work above, the biological mechanism of rheumatoid arthritis syndrome will be further studied from the regulation of LncRNA and Egr2. Methods: (1) Clinical basic study: RA patients with cold syndrome and heat syndrome will be collected,and the clinical data and immunological indexes of the patients will be measured; (2) cytological level study: preparing peripheral blood CD4 + T cells and synovial fibroblasts, RNAi method will be used to study the mutual regulation effects of LncRNA HOTAIR and Egr2 related inflammatory signaling pathway; (3) animal experiments: based on the previous work, replication CIA cold syndrome rat model, the determination of LncRNA HOTAIR and Egr2 related inflammatory signaling pathway will be measured,and then after treated by JIAWEISHAOGANFUZI Decoction , the changes of LncRNA HOTAIR and Egr2 inflammatory signaling pathway will be measured.