可调控响应性多功能抗菌表面的构建及其作用机制研究

植介入医疗器械使用过程中造成的感染是目前临床上的常见问题，给患者带来痛苦和经济负担，严重的情况下甚至导致死亡。常规的抗菌医用材料存在抗菌效率不足、失效快、功能单一等局限，从而影响了医疗器械性能的发挥。针对这些问题，本项目提出构建可调控响应性多功能抗菌表面的新概念，赋予医疗器械表面抗菌性能的同时提升其疾病治疗性能。采用响应性化学键改性天然多糖，合成具有高效抗菌性能的天然多糖衍生物，并结合生物活性分子进行修饰，提升其疾病治疗能力。基于天然多糖衍生物，利用化学方法进行可调控响应性多功能抗菌表面的构建。通过微生物学、细胞生物学、分子生物学和动物实验对其抗感染性能和疾病治疗性能进行综合评价，探索其构效关系和作用机制。通过本项目的研究，有望获得具有良好抗菌性能及治疗功能的多功能化医疗器械表面，为新型抗菌材料的设计、构建与优化提供理论依据，为突破我国医疗器械领域技术瓶颈提供新的策略与技术基础。

Medical device-induced infection is a severe problem in clinical practice, which induces significant suffering, treatment costs, and even mortality. Traditional antibacterial materials have several disadvantages, such as low efficiency, short life time, and limited functions, which have negative effects on the performances of biomedical devices. To solve these problems, this project proposes a novel concept of controllable responsive multifunctional antibacterial surfaces, which could promote the antibacterial and therapeutic properties. Natural polysaccharides will be modified with antibacterial components via responsive chemical bonds, and further functionalized by bioactive components to synthesized multifunctional polysaccharide derivatives. Controllable responsive multifunctional antibacterial surfaces will be constructed based on the polysaccharide derivatives via chemical conjugation technology. The anti-infection and therapeutic performances will be comprehensively evaluated by microbiological, cellular biological, molecular biological and animal experiments. The relationship between chemical structures and biological performances will be studied, and the acting mechanisms of multifunctional antibacterial surfaces will be explored. By this project, a series of novel medical device surfaces with highly antibacterial and therapeutic efficiencies will be acquired, which will provide theoretical guidance to break through barriers in the development of medical devices.