春季的自动脱毛，方便羊场管理，是现代肉羊育种的方向之一。脱毛是由于次级毛囊的季节性发育导致的，同时也与羊毛长度、细度等物理特性有关。目前对毛囊发育的研究较多，但针对绵羊脱毛性状的分子遗传机制研究，未见报道。项目选择脱毛性状的极端表型个体为试验对象，采集次级毛囊生长期、退行期、休止期的羊皮，采用全转录组测序测定mRNA、lncRNA、miRNA的表达量，进行差异表达分析。首先，利用差异信息进行GO、KEGG功能富集分析，趋势分析，从中寻找与脱毛性状有关的候选基因或代谢通路。然后，结合转录因子TF表达信息和数据库中TF结合位点信息，建立转录调控网络；进行lncRNA、miRNA与mRNA的关联分析，构建ceRNA调控网络。最后，对分析得到的目标分子进行后续的表达量和功能验证。此项目有助于揭示脱毛性状的分子遗传调控机制，为培育可早期脱毛的肉羊新品种奠定基础。

The auto-shedding is beneficial to management in mutton sheep production, and has been chosen as one of the important breeding aims in the modern breeding system. Shedding is due to the seasonal development of secondary follicle, but also related to wool length, fineness and other physical properties. There are many studies about hair follicle development,but the molecular genetic mechanism of shedding in sheep is unclear and needs further exploration. This project will select the sheep which have the two extreme phenotypes of shedding，cellect the wool skin of anagen,catagen and telogen of secondary follicles, and determine the expression of mRNA,lncRNA and miRNA by RNA-seq.Firstly, based on the differential expression, do the KEGG function enrichment analysis and trend analysis to find candidate genes or metabolic pathways related to the shedding trait.Secondly,construct the transcriptional regulation network by combining the differential expression of the transcription factor TF with the TF binding site information in the database, and establish the ceRNA regulation network by correlation analysis of LncRNA, miNRA and mRNA. crornas associated with mRNA analysis.Finally, the subsequent expression and functional verification will be done to make sure the condidate molecules.The project is beneficial to reveal the molecular genetic mechanism of shedding in sheep, and to provide theoretical and technical surport for the future breeding of a new mutton sheep which is characteristic of shedding.