温度是决定植物地域分布的主要限制因素。西番莲（百香果）一般在热带及亚热带地区栽培，生长要求年平均气温在18℃以上，在低温胁迫条件西番莲会遭到不同程度的伤害，而贵州喀斯特山区适生品种“平塘1号”能在零下温度条件顺利越冬。本项目拟通过不同西番莲品种的抗寒性试验，了解它们的形态结构变化和生理生化差异，解析“平塘1号”在喀斯特山区栽培的抗寒特性；同时以ICE1-CBF3-COR低温应答途径为切入点，开展“平塘1号”低温应答途径相关基因的克隆和鉴定，应用原质体瞬时表达和遗传转化技术验证其低温应答模式及生物学功能，以此来探讨“平塘1号”的分子调控机制，为喀斯特山区抗寒西番莲新品种选育及推广奠定基础。

Temperature is the main limiting factor in determining the geographical distribution of plants. Passiflora edulia is generally cultivated in tropical and subtropical regions, with the annual average temperature above 18°C. Under low temperature stress conditions, Passiflora may suffer different degrees of damage, but the suitable “Pingtang No.1” variety can be successfully wintered in the sub-zero temperature conditions of Guizhou karst mountainous areas. Through the cold resistance tests, this project will understand the morphological and structural changes and physiological and biochemical differences between different varieties of Passiflora edulia, and investigate the cold-resistance characteristics of “Pingtang No.1” variety in karst mountainous areas. Meanwhile, based on the cold-response pathway of ICE1-CBF3-COR as the focus, we will clone and identify the relevant genes. Their low-temperature response patterns and biological functions will be detected and validated by transient expression assay of protoplasts and genetic transformation experiments to explore the molecular regulation model of “Pingtang No.1”. This will provide a theoretical basis for the improvement and promotion of new cold-resistant Passiflora edulia varieties in the karst mountainous areas.