组蛋白变体H2A.Z和组蛋白甲基化修饰H4K20me2在DNA复制后的继承机制

DNA复制时，基因组根据特定的复制程序产生子代DNA；组蛋白也需要重新装配到子代DNA上，形成新生染色质。新生染色质的表观遗传信息继承对于基因组的稳定性以及细胞命运的维持至关重要。已知组蛋白变体H2A.Z能够通过调控组蛋白甲基化修饰H4K20me2帮助认证和激活早期复制起始位点，并调控基因组的复制程序。本项目旨在研究DNA复制后，H2A.Z和H4K20me2如何在子代DNA中分配，以及如何在下一轮DNA复制开始前完成恢复，从而探讨H2A.Z和H4K20me2在DNA复制后的继承机制。本研究将为理解表观遗传信息在DNA复制后的继承和动态恢复机制提供新的认识，并将进一步探讨表观遗传信息的继承对DNA复制起始位点和基因组复制程序的继承的调控。

During DNA replication, the genome is replicatied to generate daughter DNAs through defined replication timing program，and the histones also need to be re-assembled to the two daughter DNAs to form nascent chromatin. The inheritance of epigenetic information on the nascent chromatin is critical for the maintenance of genome stability and cell fate. The histone variant H2A.Z can modulate H4K20me2 levels to regulate the licencing and activation of early replication origins, and further to regulate the replication timing program.In this study, we plan to investigate how H2A.Z and H4K20me2 are distruibuted on daughter chromatin after DNA replication, and how H2A.Z and H4K20me2 are restored before next S phase.This project will offer new insights into the inheritance of epigenetic information after DNA replication, and into the inheritance of DNA replication origins and replication timing regulated by epigenetic information.