海州常山Clerodendrum trichotomum Thunb.作为“药食同源”的中药材，具有抗炎、降压、抗哮喘、抗氧化、抗HIV等多种生物活性。在课题组前期研究中发现，海州常山茎85% 乙醇提取物体外对人白血病K562细胞株有明显抑制作用，但是药效物质基础及作用机制尚不明确。项目拟以海州常山茎活性部位为研究对象，利用K562荷瘤小鼠模型，采用代谢组学的研究方法，通过观察海州常山茎醇提物对内源性代谢物谱的影响，确定海州常山抗肿瘤相关的生物标志物，寻找其主要干预的代谢通路及作用靶点，从系统生物学的角度初步阐明海州常山抗肿瘤的作用机制；对海州常山药效学数据与外源性代谢指纹图谱进行相关性分析，阐明海州常山抗肿瘤药效物质基础。构建代谢组学的内源性代谢物及外源性代谢物整合研究的模式，解决海州常山抗肿瘤作用的物质基础和作用机理不明确的问题，为进一步开发以海州常山为原料的抗肿瘤新药奠定理论基础。

Clerodendrum trichotomum Thunb. is homologous plant food medicine. It has many biological and pharmacological activities, such as anti-inflammatory, anti-hypertension, anti-asthmatic, antioxidation and anti-HIV virus. The experiments on anti-tumor of C. trichotomum in vivo has not been reported although C. trichotomum can inhibit the growth of a wide range of cancer cells in vitro. The material base for efficacy and action mechanism of anti-tumor still remains unclear. Previous research shows that the 85% ethanol extract from C. trichotomum has significant inhibiting effect on proliferation of leukemia K562 cell line in vitro. The studies are planned to be conducted focusing on the effective ingredients of C. trichotomum and based on metabolomics and mice bearing neoplasm (K562 cell line). The following research work will figure out the endogenous biomarkers and their main metabolic pathways as well as their intervention targets through studying the effect of effective ingredients on the expressions of endogenous metabolites of mice bearing neoplasm (K562 cell line) . The mechanism of action of anti-tumor of C. trichotomum will therefore be elucidated from the perspective of systems biology. At the same time, the correlation between pharmacodynamic data and exogenous metabolic fingerprints of C. trichotomum will be analysised, and the efficacy material base of anti-tumor will be clarified. In addition, the systematic study of chemical constituents of C. Trichotomum will be carried on. The project will provide a integrated research mode of metabonomics by discussing the endogenous and exogenous metabolites. The results indicate that the unclear problems of material basis and mechanism will be solved, which is the theoretical basis in the further development of anticancer drugs from C. trichotomum.