CircRNA1397竞争性调控miR-206抑制软骨细胞凋亡在委陵菜酸治疗骨关节炎中的作用机制

骨关节炎（OA）发病率高但尚无特效干预手段，发病机制复杂有待深入研究。我们前期工作发现委陵菜酸（TA）是OA的有效治疗药物，进一步研究发现OA软骨细胞circRNA1397表达明显降低，而TA可引起circRNA1397表达上调。生物信息学分析证实circRNA1397靶基因包括miR-206，同时发现miR-206与Bcl-2 mRNA 3'UTR存在结合位点。细胞实验证实miR-206可抑制Bcl-2表达。据此我们推测TA可能通过circRNA1397竞争性结合miR-206，上调Bcl-2从而抑制软骨细胞的凋亡。在本研究中，我们将利用siRNA、质粒转染、荧光素酶实验及Tunel染色技术等方法，分别在临床组织、动物和细胞水平开展研究，明确circRNA1397在OA中的功能，并基于非编码RNA与mRNA之间网络揭示TA治疗OA的分子机制，为OA防治提供新靶点与新策略。

The incidence of osteoarthritis (OA) was high but there was no special intervention. And its complicated pathogenesis need further research. We found that Tormentic Acid (TA) were effective drugs for the treatment of OA in our previous researches, and we also found that the expression of circRNA1397 down-regulated obviously in OA chondrocytes, however, the expression of circRNA1397 up-regulated in the group of TA. Bioinformatics analysis confirmed that the target gene of circRNA1397 included miR-206, which existed combining site together with Bcl-2 mRNA 3’UTR. Furthermore, cell experiments demonstrated that miR-206 inhibited the expression of Bcl-2. According to these grounds, we speculated TA competitive binding miR-206 through circRNA1397, which up-regulated the expression of Bcl-2 so as to suppress the apoptosis of chondrocyte. In our study, we will take advantage of siRNA, plasmid transfection, luciferase experiment and Tunel staining technique and so on, which will carry out in the levels of clinical tissues、animal experiment and cell experiments in order to identify that the function of circRNA1397 in OA. What is more, on account of the web of non-coding and coding RNA, we will reveal the molecular mechanisms of TA treatment of OA, in order to provide new targets and new strategy for prevention and treatment of OA.