类风湿关节炎(RA)的发生发展涉及多种信号通路调控，研究表明糖代谢影响T细胞的功能，与RA的发病密切相关。补肾抗风湿方药-寒痹康汤是治疗RA疗效确切的临床经验方，项目申报人前期研究显示，该方可以抑制RANKL和RANK的蛋白和基因表达，进而抗RA骨质侵蚀。本项目在此基础上，以Notch调控 PI3K/Akt/mTOR信号通路调节RAT细胞糖代谢为切入点。以CIA大鼠为研究对象，留取膝关节滑膜，采用流式细胞术、RT-PCR、Western-blot、免疫组织化学法等技术检测治疗前后Notch及PI3K/Akt/mTOR信号通路相关的Notch受体、Notch配体、CSL-DNA结合蛋白、PI3K/Akt/mTOR基因及蛋白表达，检测Glut1、c-Myc、HIF1以及多种糖酵解关键酶的变化，分析上述指标变化，从RAT细胞糖代谢角度研究寒痹康汤对RA的影响，从而阐明其治疗RA的作用机制。

The occurrence and development of rheumatoid arthritis (RA) involves a variety of signaling pathways. Studies have shown that carbohydrate metabolism affects the function of T-cells and it is closely related to the pathogenesis of RA. The nourishing kidney and Anti-rheumatoid prescription medicine-Hanbikang Tang has a definite therapeutic effect on the treatment of RA,and the project applicant preliminary studies have shown that the formulas can inhibit RANKL and RANK protein and gene expression, and thus anti-RA bone erosion.On the basis of this study, by adjusting T-cell carbohydrate metabolism in RA based on Notch regulation of PI3K/Akt/mTOR signal pathway as a breakthrough point.Take the synovial membrane of the knee in CIA rats , using flow cytometry, RT-PCR, Western-blot ,immunocytochemistry to detect the Notch and Notch receptor, Notch ligand, CSL-DNA binding protein, PI3K/Akt/mTOR gene and protein expression related to PI3K/Akt/mTOR signaling pathway before and after treatment.And it also detects changes in Glut1, c-Myc, HIF1 and various key glycolytic enzymes. Analysis of the above indicators, from the perspective of carbohydrate metabolism of RA T-cells to study the effect of Hanbikang Tang on RA, thus clarifying its mechanism of action in the treatment of RA.