谱系重编程获得的干/祖细胞或成熟细胞可直接应用于临床，但目前谱系重编程获得的细胞在数量上的不足以及在功能上的不完善限制了其在生物医药领域的广泛应用。我们在肝干细胞谱系重编程的研究中发现染色质重塑因子Snf5具有显著提高肝干细胞谱系重编程效率的作用，但Snf5能否增强谱系重编程获得的肝干细胞的功能及其发挥作用的机制仍需进一步的研究。由于对谱系重编程机制的阐明能够促进谱系重编程技术的完善和发展，因此，本研究拟通过对Snf5在肝干细胞谱系重编程过程中的作用及机制的研究，阐明肝干细胞谱系重编程过程中染色质结构变化、DNA甲基化及组蛋白修饰等表观遗传的变化规律，有助于形成基于染色质重塑等表观遗传修饰的方法促进肝干细胞谱系重编程的效率及增强细胞功能的谱系重编程新策略。

The stem/progenitor cells and mature functional cells achieved by lineage reprogramming can be directly used in clinic. But the deficiency in quantity and quality of lineage reprogramming cells limit its application in the field of biology and medicine. In previous study, we found that the chromatin remodeling factor Snf5 could significantly improve the hepatic stem cells reprogramming efficiency. However, whether Snf5 can enhance the functions of induced hepatic stem cells by lineage reprogramming and its mechanism are needed to be further investigated. The elucidation of the mechanism of lineage reprogramming could promote the development and improvement of lineage reprogramming. Hence, this study intends to further investigate the effections and mechanism of Snf5 in the process of hepatic stem cell lineage reprogramming. The epigenetic change rules of chromatin structure, DNA methylation and histone modification in the process of hepaic stem cell reprogramming will be clarified. The Elucidation of the mechanism of lineage reprogramming will be conductive to establish a new strategy by epigenetic modification of chromatin to improve the efficiency of reprogramming and the enhancement of cell function in the hepatic stem cell lineage reprogramming.