本课题根据中医"异病同治"原则，在既有文献和自身前期研究基础上提出ASO的发病机制与老年肾阳虚血管细胞内钙超载相关的假说。选临床有效方蠲脉Ⅰ号的温阳拆方进行实验：（1）整体实验：分正常组、模型组、假手术组、蠲脉Ⅰ号温阳拆方治疗组、蠲脉Ⅰ号全方治疗组，用兔ASO模型接受治疗，检测其血液流变学、生化、NO、血栓素、6酮前列腺素等指标和血管细胞内钙离子变化以及血管细胞钙调蛋白的表达，对比诸项指标与血管组织钙调蛋白的表达；（2）体外实验：分正常对照组、血管紧张素组、血管紧张素+蠲脉Ⅰ号温阳拆方组、血管紧张素+蠲脉Ⅰ全方组4组，观察各组对抑制血管紧张素诱导的体外血管平滑肌细胞增殖实验的影响，检测其增殖周期和凋亡变化，激光共聚焦显微镜动态观察细胞内钙，对比该方对血管平滑肌细胞中钙调蛋白的活性影响。以此印证假说的科学性，为中医早期防治ASO提供新的理论依据,并为研发临床新药奠定基础。

The subiect according othe principle of Chinese medicine"treating difference disease with the same method",ASO pthogenesis of senile kidney yang vascular cells related to intracellular calcium ocerload hypothesis proposed in the existing literature and their own pre-study basis.JM No.1 WYCF selected clinically effective for the experiment:(1)the overall experiment:sub-mormal group,model group,sham operation group,JM No.1 WYCF group,JM No.1 group,Treated with rabbit ASO model.Detection of blood rheology,biochemical NO,thromboxane,6-keto prostaglandin and other indicators and changes in intracellular calcium in vascular cells and vascular cell expression of calmodulin.Compare the various indicators and the vascular tissue exppression of calmodulin;(2)In vitro experments:Normal control group;AngⅡgroup;AngⅡ+JM No1.WYCF group;AngⅡ+JM No.1group;Each group were observed to inhibit the in vitro VSMC proliferation induced by Ang II experiment,Detection of VSMC proliferation cycle and apoptosis changes,The laser scanning confocal microscope dynamic observation of intracellular calcium, compared to the JM No.1WYCF activity effects on VSMC CaM.This confirms the hypothesis of the scientific, and provide a new theoretical basis for traditional Chinese medicine early prevention of ASO, and lay the foundation for the clinical development of new drugs.