基于木质牙签离子化技术的代谢组学平台的建立及其在川贝母基原鉴定和亲缘学研究中的应用

本课题以多来源中药材的鉴定和亲缘关系为出发点，以川贝母为研究对象，针对不同基原种川贝母等同用药的合理性研究的不足，结合课题组前期研究的基础，拟将植物代谢组学的方法引入多来源中药材的鉴定及药用亲缘学研究。拟解决的关键科学问题：首先，建立木质牙签直接取样离子化技术（WT-ESI-QTOF/MS），解决中药粉末样品、提取物等样品的直接分析的难题；然后建立基于WT-ESI-QTOF/MS的植物代谢组学研究平台，解决多来源中药材快速鉴定的难题及真伪混合样品的鉴定难题；进而将该植物代谢组学研究平台引入川贝母的药用亲缘学研究，找出标志性代谢产物的同时，阐释六种川贝母的“亲缘关系—化学成分—疗效”之间的内在联系，为其等同用药的合理性提供数据支持。本项目为多来源中药材的鉴定和药用亲缘学关系的研究提供了方法学示范。

The project will focus on the plant metabolomics-based identification and pharmacophylogenetic study of Fritillariae Cirrhosae Bulbus (FCB). Firstly, wooden-tip electrospray ionization mass spectrometry (WT-ESI-QTOF/MS) and a strategy will be developed to rapid identification of different species of FCB. Then, the WT-ESI-QTOF/MS method is used for global analysis of the primary metabolites and some secondary metabolites in FCB. Subsequently, we use multivariate statistical analysis methods to deal with the normalized data, finding out and identifying the marker metabolites. In the same way, we can explore the genetic relationship between six species of FCB. Finally, we evaluate the biological activity in antiasthmatic of these six species of FCB. By comparing and analyzing the difference in biological activity and chemical constituents, we clarify the inherent connections between pharmacophylogenetic relationship, chemical constituents, and biological activity. This study introduces the plant metabolomics in pharmacophylogenetic study, providing a new tool for finding the pharmacophylogenetic relationship.