两种肺腺癌细胞体内外放射后残留细胞增殖迁移及干细胞变化的比较性性研究

放疗后肿瘤的复发转移是肺癌放射治疗中难以解决的问题。研究发现一些肿瘤细胞经放化疗后残留细胞的生长、侵袭转移及肿瘤干细胞特性等发生改变，但变化并不一致。云南宣威肺腺癌XWLC-05和普通人肺腺癌A549细胞存在固有的放射敏感性差异，且与PI3K/AKT通路活性差异、细胞凋亡、细胞周期分布及DNA损伤与修复有关。不同照射可能会对两种残留肺癌细胞的生物学行为造成不同的影响。本项目旨在通过不同剂量X线照射后的XWLC-05和A549残存细胞及小鼠移植瘤放射敏感性、细胞增殖、迁移、肿瘤干细胞比例、细胞凋亡、周期分布、DNA损伤与修复、PI3K/AKT通路活性及相关基因、蛋白的表达变化，体内外对照研究两种肺癌细胞经不同照射后残存细胞生物学行为的改变及差异，并探讨其发生机制，以期对肺癌的放射及综合治疗带来指导。

Tumor recurrence and metastasis are the problems of lung cancer radiotherapy. It was reported that the changed bio-effects were found on some residual cells after radiotherapy and chemotherapy. But the changes were inconformity. The inherent radiosensitivity between Yunnan Xuanwei lung Cancer cell XWLC-05 and lung adenocarcinoma cell A549 was different. And it was related with PI3K/AKT activity, cell apoptosis, cell cycle distribution, DNA damage and repair. It is suggested that different radiation may take the different bio-effect on two kinds of residual lung cancer cells. In the study, we will focus on the residual cells of XWLC-05 and A549 cells after different dose and different fraction in vivo and in vitro. We will investigate the changes of cellular radiosensitivity, proliferation, metastasis，tumor stem cell rates, apoptosis, cell cycle distribution, DNA damage and repair, the activity of PI3K/AKT pathway. And we will detect the expression of related genes and proteins investigate. The study will analyze the changes and difference of bio-effects on XWLC-05 and A549 cells after different radiation and explore the related mechanism. It will bring the guidance for lung cancer therapy.