云南边境口岸基孔肯雅病毒的流行特征及其跨境传播规律研究

基孔肯雅热是由基孔肯雅病毒(CHIKV)引起，经伊蚊叮咬导致的一类急性传染病。CHIKV感染导致慢性化关节炎，目前没有针对性的疫苗和有效药物，成为全球关注的重大公共卫生问题。近年来，CHIKV在东南亚国家频繁爆发。云南与东南亚接壤，且云南地区伊蚊分布广泛。近期，我们的研究提示CHIKV已在云南地区形成了由跨境人群—伊蚊—本地人群之间的传播，为该地区CHIKV防控带来巨大挑战。基于此，本研究拟通过对云南边境口岸和本地感染人群的样本收集，CHIKV流行疫区伊蚊的采集，对这些样本通过CHIKV病毒基因型、亚型和基因突变研究，阐明云南地区CHIKV的流行特征；结合Genbank资源通过病毒遗传进化分析，揭示该病毒在输入性人群—伊蚊—本地感染者之间病毒的演化规律；进一步利用网络关联分析技术，绘制三者间病毒传播的网络。进而系统性阐明CHIKV跨境传播的机制为云南地区CHIKV的防控提供重要科学依据。

Chikungunya fever is an acute infectious disease caused by chikungunya virus (CHIKV). Chronic arthritis is caused by CHIKV infection. At present, there is no targeted vaccine and effective treatment drugs, resulting in a major public health issue of global concern. Recently, CHIKV has broken out frequently in Southeast Asian. Yunnan borders Southeast Asian countries, and Aedes mosquitoes are widely distributed in Yunnan. Recently, our result indicates that CHIKV has been spread among cross-border population, Aedes mosquito and local population in Yunnan, bringing great challenges to the prevention and control of CHIKV in here. In this study, we intends to collect the blood samples isolated from the cross-border population, Aedes mosquito and local population with CHIKV infection in Yunnan, and clarify the epidemic characteristics of CHIKV in Yunnan through the study of genotype, subtype and gene mutation of CHIKV. Further, through the analysis of virus genetic evolution combined with GenBank database, it is revealed that evolution of CHIKV among the imported population, Aedes, and local infectors. In addition, we character the network structure of virus transmission among them. In this study, we intend to systematically clarify the mechanism of CHIKV cross-border transmission to provide an important scientific basis for the prevention and control of CHIKV in Yunnan.