针刺对脑卒中肢体痉挛大鼠GABAA-BDNF-TrkB-突触可塑性蛋白信号通路的影响

探讨脑卒中肢体痉挛状态发生、变化机理，并开辟有效的疗法，是当前急需解决的关键问题。研究表明脑卒中后肢体功能的恢复与突触可塑性相关，突触可塑性与BDNF-TrkB 信号相关，而本课题组以往临床、动物研究结论显示针刺缓解脑卒中肢体痉挛状态的机理与调节脑内GABA受体的mRNA表达水平有关，因此提出假说：针刺治疗脑卒中肢体痉挛状态可能与调节脑内的“GABAA-BDNF-TrkB-突触可塑性蛋白”信号通路相关。本研究拟以脑卒中肢体痉挛状态大鼠模型为受试对象，运用动物神经行为学、电生理、超微透射电镜、免疫组化及荧光实时定量PCR技术方法，从细胞和分子水平阐述针刺缓解脑卒中肢体痉挛状态的作用机理，是通过调节大脑皮质、海马的GABAA-BDNF-TrkB-突触可塑性蛋白信号通路而实现的，为针刺治疗脑卒中肢体痉挛状态的临床推广应用提供实验依据。

To investigate the limb spasticity of cerebral stroke, change mechanism, and develop effective therapy, is a key problem needed to be solved. Research shows that stroke recovery and synaptic plasticity after limb function related to synaptic plasticity and BDNF-TrkB signaling, and the research group to clinical and animal study showed that the level of mechanism of acupuncture ease of limb spasticity in stroke and regulation of GABA receptor in the brain mRNA expression, thus put forward the hypothesis: the acupuncture treatment for stroke spasticity may be related to the regulation of brain GABAA-BDNF-TrkB- synaptic plasticity protein signal pathway. In the present study of limb spasticity in stroke model rats as subjects, the use of animal behavioral, electrophysiological and ultrastructural transmission electron microscopy, immunohistochemistry and fluorescence quantitative real-time PCR technique, expounds acupuncture relieve stroke from cellular and molecular level The mechanism of limb spasticity is by regulating the cerebral cortex, hippocampus GABAA-BDNF-TrkB- synaptic plasticity protein signal pathway, for acupuncture treatment of stroke spasticity clinical application to provide experimental basis.