基于JAK/STAT通路探讨温阳通络针灸法干预缺血缺氧脑瘫幼鼠的作用机制研究

小儿脑瘫是一个高社会和高经济负担的疾病。出生窒息/缺血缺氧是其发病的高危因素。我们前期应用温阳通络针灸治疗小儿脑瘫，通过四肢肌力、运动控制能力变化的评价发现有显著疗效，但其作用机理尚不明确。JAK/STAT信号转导通路已被证实是调节脑缺血缺氧后细胞因子反应的重要通路。基于“络脉-血管微循环”理论，我们提出假设：温阳通络针灸治疗缺血缺氧脑瘫的机制可能是调控Janus激酶信号传导和转录激活蛋白，介导JAK/STAT通路，改善脑部微循环，参与脑发育过程。本项目通过温阳通络针灸治疗缺血缺氧脑瘫幼鼠模型研究，应用行为学、炎症因子ELISA法、脑血流变、组织病理、免疫组化、WesternBlot、荧光脑血管密度等方法，观察针刺对脑血流、脑血管重建、JAK2、STAT3的影响与调控，探索温阳通络针灸治疗缺血缺氧型小儿脑瘫的作用路径及靶点。为阐明温阳通络针灸治疗缺血缺氧型小儿脑瘫的临床机制提供科学依据。

Pediatric cerebral palsy is a disease with high social and high economic burden. Birth asphyxia /hypoxic ischemic encephalopathy are the main risk factors for cerebral palsy in children. Our previous study applied Wenyang Tongluo acupuncture to treat children with cerebral palsy. It was found that the muscle strength and motor control ability of the limbs were significantly improved, but the mechanism of action was not clear. The JAK/STAT signal transduction pathway has been shown to be an important pathway regulating cytokine responses following cerebral ischemia and hypoxia. Therefore, we hypothesized that the mechanism of "Luo Meridian-Microvessels" of hypoxic cerebral ischemia may be through the regulation of Janus kinase signaling and transcriptional activator protein, mediating JAK/STAT pathway, improving brain microcirculation and participating in brain development. The project used acupuncture and moxibustion for treating hypoxic-ischemic cerebral palsy in young rats by ways of behavioral, inflammatory factor ELISA, cerebral hemorheology, histopathology, immunohistochemistry, Western Blot, fluorescent cerebrovascular density and other methods to observe acupuncture and the effects and regulation of cerebral blood flow, cerebral revascularization, JAK2, STAT3, and the role and target of Wenyang Tongluo acupuncture in treating cerebral palsy in rats with hypoxia-ischemia to provide a scientific basis for clarifying the clinical mechanism of Wenyang Tongluo acupuncture in the treatment of cerebral palsy in children with hypoxia-ischemia.