动物药的鉴定目前主要依据宏观或微观的外在可视特征、小分子或DNA等，存在一些固有的局限性。本项研究以MALDI-TOF/TOF-MS为核心技术，并结合二维电泳、二维纳流液相色谱和肽序列从头测序算法等手段，选择僵蚕、鹿角、鹿茸、阿胶和龟甲为模型药物，解析多基源、复合体类、角类、动物甲壳类和胶类动物药药材与饮片中的标志蛋白质和多肽等，以作为它们的特征识别物，并以其含有的特异性多肽片段作为鉴定的直接依据。本项研究可为动物药的专属性鉴定构建一条新的研究途径，建立关键的技术，对确保动物类中药药材和饮片的质量，从而保证用药的安全性、有效性具有重大的应用价值；同时，本研究可促进蛋白质组学、生物信息学与中药鉴定的有机结合，有着重要的学术意义。

Presently, authentication of animal-derived medicines is primarily based on the macro or micro external visual characteristics, small molecules or DNA, etc., and some undesired inherent limitations exist. In this proposal, MALDI-TOF/TOF-MS as the key technology, and two-dimension electrophoresis, two-dimension nanoflow liquid chromatography and de novo sequencing are incorporated for the elucidation of signature proteins and peptides in multi-source, complex, horns, animals crustaceans and glue-like animal-derived medicines with Bombyx Batryticatus, Cervi Cornu, Cervi Cornu Pantotrichum, Asini Corii Colla and Testudinis Carapax ET Plastrum as models. These identified proteins and peptides can be used as their specific markers, and the intrinsic unique peptide fragment in the markers would be the direct evidence for the authentication of these animal-derived medicines. The key technology established could be a new approach for the specific authentication of animal-derived medicines, with the intention to help ensure the quality, the safety and the effectiveness of the medicines in their raw material and ready-in-use form. At the same time, the study has an important academic significance to promote the seamless integration of proteomics, bioinformatics and Chinese medicine authentication.