基于CRS的“怒伤肝”大鼠即早基因表达与信号传导通路机制研究

课题对大鼠昼夜颠倒环境、破坏双侧膈区后怒行为、双后肢立式束缚三种“怒伤肝”造模方法，采用Openfield评价，完善“怒伤肝”模型；通过检测“怒伤肝”大鼠脑区即早基因的表达及信号传导通路中CRH、ACTH、ACTH受体信使RNA的表达变化以及免疫功能的改变，用逍遥散加以干预调节，研究在应激及中药干预不同时段脑区即早基因的表达及信号传导通路效应机制；覆盖广义粗糙集将粗糙集等价关系确定的划分推广为覆盖，是对粗糙集理论的一种重要扩展。课题采用先进的基于粗糙集的数据挖掘技术，分析“怒伤肝”大鼠即早基因的表达及信号传导通路效应间的关联关系以及变化规律，根据数据挖掘的结果，结合中医证候专业知识，对所提取的证候分类知识进行合理的解释与评价，建立一套基于覆盖粗糙集数据挖掘技术和中医证候研究相关的科学评价体系。

According to the three kinds of "anger injured liver" model method（Rats upside down the environment day and night,Wrath after destroying bilateral diaphragmatic area，Vertical bondage of double hind legs）, the “Openfield” evaluation was used to improve the "anger injured liver" model;by detecting the expression of the early gene in the brain region of rats with "anger injury liver" and the expression changes of CRH, ACTH and ACTH receptor messenger RNA and the changes of immune function in the signal transduction pathway.With the intervention of Xiaoyao Powder, the expression of early gene and the effect mechanism of signal transduction pathway in the brain region of different periods of stress and Chinese medicine intervention were studied.It is an important extension to the rough set theory that the generalized rough sets cover the partition of the equivalence relation of rough sets as coverage.Based on rough set data mining technology, we analyzed the relationship between the expression of early genes and the effect of signal transduction pathways, and the changing rules on "anger injured liver" rats.According to the results of data mining, combined with the knowledge of TCM syndromes, we can reasonably interpret and evaluate the extracted syndromes knowledge, and establish a scientific evaluation system based on covering rough set data mining technology and TCM syndrome research.