载脂蛋白H在抗磷脂抗体综合症中作用的细胞与分子机理

载脂蛋白H在抗磷脂抗体综合症的作用的研究正深刻影响对这一自身免疫病的认识颐墙訟PS中该蛋白结构功能的变化，抗该蛋白抗体的结构功能，该蛋白及其抗体对血苣谄は赴饔眉捌涫芴搴托畔⑼揪叮玫鞍准翱固逵肷鱿赴慕岷霞耙鸬南赴峁构δ艿母谋湔馑姆矫婵寡芯浚酉赴肿铀缴仙罨韵喙刈陨砻庖卟〉娜鲜恫⒂幸嬗诹俅彩导

Antiphospholipid syndromes（APS）are serious autoimmune diseases. b2-glycoprotein I （b2-GPI）or apolipoprotein H （ApoH）. In recent years, interest in b2GPI increased dramatically because it has been proposed to be most clinically relevant in patients with anti-phospholipid syndrome (APS). .The present study are shown bellow:.1．To investigate polymorphisms of human serum b2-glycoprotein I in about 500 healthy Chinese and 52 APS positive by isoelectroric focusing analysis. It indicated that although for APS patient, the ApoH allele frequencities do not significantly alter, some abnormal changes of serum ApoH in gene expression and molecular structure may occur, resulting in the alteration of antigenicity..2．Anticardiolipid antibody (ACA) in APS patients' serum displayed remarkable anti-b2-GPI activity. It is proposed that the ACA-targeted antigenicity was involved in alterated b2-GPI, which might cause 'antigen-drive' immunoreaction. .3. For first time indicated that there was a Fc-binding factor in APS patients' serum, which was identified with anti-b2-GPI antibody as well as ACA..4. For first time showed that the plasma soluble protein b2-glycoprotein is a cofactor or activator of tPA to significantly augment the tPA-dependent plasminogen activation. The specific ability of b2GP can be inhibited markedly by anti-b2GP antibody and was b2GPI dose-responsive, and that the further activation by plasmin may provide a nevol positive feedback mechanism of intrinsic fibrinolysis.The complex prothrombosis and anti-coagulation in APS patient' vascular system was explained in view of this novel activation pathway of tPA-dependent plasminogen system.5. To demonstrate the importance of the formation of b2-GPI-antib2-GPI antibody-annexin II-anti annexin II antibody complex in the cellular mechanism of APS. To investigate bioenergics in apoptosis, the relation between BcL-2 gene and telomerase activities in apoptosis, the anti-apoptosis of collagen..