腹膜间皮细胞(PMCs) CD40/CD40L相互作用激活介导产生免疫细胞因子是腹膜损伤后炎症-纤维化级联反应的触发点，在腹膜损伤后继发多种病理生理改变中起关键作用。有证据显示在CD40/CD40L信号激活介导下，PMCs呈现出对粘连形成的闸门效应。申请者在长期观察活血通腑方防治术后腹腔粘连时程效应基础上，提出该方效应机制可能与调控CD40/CD40L通路及刺激炎症因子所致级联状态进程有关，设计:基于CD40/CD40L共刺激通路开展该方防治大鼠术后腹腔粘连作用机制研究。拟围绕CD40/CD40L信号及炎症调控因子，用免疫荧光组化、Western blotting 和RT-PCR 等技术手段系统研究活血通腑方对整体动物术后及体外PMCs CD40/CD40L共刺激信号的干预作用，发现其介导效应的信号通路，阐明其防治术后腹腔粘连分子作用机制，明确药物新的作用靶点，为临床推广应用提供科学依据。

The interaction of CD40 with its ligand (CD40L) in peritoneal mesothelial cells (PMCs) mediates activation of some immune cytokines, which is the triggers for inflammation-fibrosis cascade reaction, playing a crucial role in the various pathological and physiological changes after peritoneal injury. More evidences demonstrate that PMCs exert the gate for adhesion formation mediated by the activation of CD40/CD40L co-stimulating signal. Based on our findings in long-time studies that effective clinical prescription Blood-activating and Organ-purging formula regulated PMCs function to prevent postoperative peritoneal adhesion at time-effect dependent manner after surgery, we suggested that these effects of this prescription may be associated with its regulation of PMCs CD40/40L co-stimulating signal pathway and inflammatory factor-induced the cascade reaction. Therefore, this project will investigate the mechanisms by which Blood-activating and Organ-purging formula prevents postoperative peritoneal adhesion mediated by PMCs CD40/CD40L co-stimulating signal pathway. To further understand this design, this project will investigate the effects of Blood-activating and Organ-purging formula focusing on CD40/CD40L co-stimulating signal pathway and inflammatory regulation factors in PMCs of rats after surgery, as well as in vitro model of PMCs by the methods of immunofluorescence histochemistry, Western blotting and Real-Time PCR, respectively. This projest will find the signal pathway mediated by Blood-activating and Organ-purging formula in this condition. These results from this study will reveal the molecular mechanisms of Blood-activating and Organ-purging formula and new targets of drug action, as well as provide the scientific evidences for clinical application.