人毛乳头细胞（DPC）具有毛囊诱导功能，是构建人组织工程毛囊重要的种子细胞。但是，DPC经体外常规二维培养后会逐渐丧失诱导能力。正常人体内的DPC聚集呈球状，周围被丰富的细胞外基质包裹（ECM）。层层自组装(LBL)技术被广泛用于构建细胞纳米仿生ECM及微环境。我们在近期研究中以LBL技术成功构建出了鼠单个DPC的仿生纳米超薄ECM包裹模型(LBL-DPCs)，为单个DPC提供了合适的微环境。此外，我们在包裹层中载入特定生长因子后能一定程度上增强DPC的毛囊诱导能力。在后续实验中，我们基于化学交联技术构建出了仿生化的鼠LBL-DPCs多细胞球状微组织，这一定程度上模拟了DPC体内微环境。基于此，我们拟采用 LBL技术构建人DPC纳米超薄ECM，并以化学交联技术构建出仿生人DPC微组织，此后与人毛囊干细胞三维共培养，以期重建出人组织工程毛囊。

The dermal papilla cell (DPC) is the most important cell in construction of tissue engineering hair follicle(HF), due to its hair-inducing capacity. However, the inductive ability of DPCs tends to gradually lose during passaging once they are cultured in traditional 2D culture in vitro. In vivo, DPCs are physiologically embedded in extracellular matrix (ECM), forming into a multi-cellular spheroid tissue that exhibiting a unique 3D organization. Since Layer-by-layer self-assembly (LBL) is an ultrathin ECM nano-fabrication technique to create a 3D biomimetic microenvironment. Recently, we have developed a single mouse DPC-based LBL encapsulation model (LBL-DPC) by utilizing the LBL technique, and DPC hair-inductivity was enhanced when defined growth factor was loaded into the LBL nano-structure. In our latest work, we have preliminarily established a biomimetic model of mouse LBL-DPCs multicellular spherical microtissue based on chemical crosslinking. Thus, we want to construct nanometer ultra-thin ECM for human DPC for LBL-DPCs microtissue, which co-culture with human HF stem cell to reconstruct bionic microenvironmentand tissue engineering HF. It will not only provides a valuable platform for exploring the biology and mechanism of human HF, but also confers a potential therapeutic strategy for treating hair loss diseases.