微阵列表面修饰3D纳米支架引导神经干细胞定向迁徙修复脊髓损伤

近年来生物材料表面修饰的研究发现，微阵列技术制备具特定界面构型的ECM支架，对细胞的生长迁徙具特殊引导作用。我们之前的研究已构建具特定可控界面构型的二维生物支架，并成功地引导了神经干细胞的定向生长、迁徙。这对引导细胞穿越脊髓损伤区域修复神经功能具重要意义。 但研究中我们也发现，单纯的二维界面-细胞培养难以模拟体内复杂的三维环境，这也是可控界面构型二维支架难以深入进行体内实验的最大障碍。而最新的高精度3D打印技术的发展为界面生物学的探索性研究提供了有力工具，其可将二维平面的细胞-材料界面研究，深化为可控三维立体空间细胞-界面生物学研究。 因此，我们拟通过对材料界面形态的三维优化设计，利用高精度3D打印技术及具良好生物物理特性的新型生物材料，制备具特定可控界面构型特征的三维ECM支架，以更好的趋向性引导神经干细胞的定向生长、迁徙，探索通过可控界面构型生物支架修复脊髓损伤的新途径。

During the research of surfaces modification to the bio-scaffold, the scientist find by prepare a controllable micropatterned surfaces, which can limit the living microenvironment of the cells, and to guide the growth and migration of the cells. We have successfully construct the bio-scaffolds with controllable micropatterned surfaces to promote the directional growth of neural stem cells, which was of great importance for guide the cells through the damaged areas and for the fuctional regeneration of the injuryed spinal cord. But we found during our research, the two-dimensional surface-cell culture can not imitate the complicated three dimensional microenvironment in vivo, which was also to be the most difficult obstacles for the in vivo application. The newly development accuracy of three dimensional print technique provide useful tools for the interface biology research, which can deepen the two-dimensional surface-cell culture research to a three dimensional studies. So, we prepare for a better design of the micropatterned surfaces in a three dimensional structures, with the methods of three dimensional print technique and newly bio-scaffolds, to prepare the three dimensional bio-scaffolds with controllable micropatterned surfaces. In order to better induction the neural stem cells growth and migrate at specified direction, and to explore this new methods with controllable micropatterned bio-scaffolds for spinal cord repairing.